We narrowed to 7,293 results for: 11

1. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

   Plasmid

   #52895

   Purpose

   Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Zipp.-L-IFP-F[1]
   

   Tags

   L-IFP-F[1]

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pBstSK+IRES-V5-IPpoI (mutant)

   Plasmid

   #206322

   Purpose

   A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

   Depositor

   Brian McStay

   Article

   van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

   Insert

   IRES element-V5 epitope tag-mutated IPpoI open reading frame
   

   Use

   Mrna production

   Tags

   V5-IPpoI fusion protein (mutant version)

   Available Since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYCDuet_D27NDHFR_WTTS

   Plasmid

   #91244

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D27N

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYCDuet_F31YG121VDHFR_WTTS

   Plasmid

   #91229

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F31YG121V

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYCDuet_G121VDHFR_WTTS

   Plasmid

   #91233

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G121V

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK2_006

   Plasmid

   #123701

   Purpose

   Encodes pSV40 as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pSV40
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. deltaPYD-NLRP11.MYC

   Plasmid

   #131197

   Purpose

   mammalian expression

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

   Insert

   deltaPYD-NLRP11 (NLRP11 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   deletion of the pyrin domain (amino acids 1-124 m…

   Promoter

   pCMV

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-NES-ZapCmR1.1

   Plasmid

   #59014

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
   

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCfB1975 (pUC19-PTEF1-PPGK1)

   Plasmid

   #73296

   Purpose

   Example plasmid containing a bidirectional promoter

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   pTEF1-pPGK1
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/3xmyc_NLRP11-LRR

    Plasmid

    #131200

    Purpose

    mammalian expression

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

    Insert

    LRR of NLRP11 (NLRP11 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains only the leucine-rich repeats (LRRs), am…

    Promoter

    pCMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4 mIL-17 1.1kb promoter

    Plasmid

    #20125

    Depositor

    Warren Strober

    Article

    Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

    Insert

    mIL-17 (Il17a Mouse)
    

    Use

    Luciferase

    Available Since

    Jan. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

12. LifeAct-TagBFP-DiB-RM

    Plasmid

    #168782

    Purpose

    Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    LifeAct-TagBFP-DiB-RM
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. BDS-2 3x MUT (p53 binding site)

    Plasmid

    #16516

    Depositor

    Bert Vogelstein

    Article

    Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

    Insert

    p53 binding site (SFN Human)
    

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant p53 binding sites

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCFJ907

    Plasmid

    #44473

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, [4-3] Gateway
    

    Use

    Gateway destination vector

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. MEIS1A-pSP65

    Plasmid

    #21014

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

    Insert

    MEIS1A (Meis1 Mouse)
    

    Use

    In vitro tnt

    Tags

    Flag

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pONSY-mCherry

    Plasmid

    #111874

    Purpose

    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK3b_004

    Plasmid

    #123785

    Purpose

    Encodes mAzamiGreen as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mAzamiGreen
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATF4 13: 5’ATF4.uORF2AUA.GFP

    Plasmid

    #21860

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    AUG codon of uORF2 was converted to AUA

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sha2Cas9

    Plasmid

    #192140

    Purpose

    Expresses Sha2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    Sha2Cas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpeCas9

    Plasmid

    #192139

    Purpose

    Expresses SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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