We narrowed to 7,519 results for: Lif

1. Flrt2-Fc-His

   Plasmid

   #72074

   Purpose

   Expresses the extracellular region of the FLRT2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Flrt2 (Flrt2 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB1N-mScarlet-LZ-LOV2

   Plasmid

   #197419

   Purpose

   Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Use

   Tags

   A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

   Plasmid

   #87827

   Purpose

   Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter, tetracycline operator

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Anti-PICK1 [L20/8R]

   Plasmid

   #128630

   Purpose

   Mammalian Expression Plasmid of anti-PICK1 (Human). Derived from hybridoma L20/8.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-PICK1 (Human) recombinant mouse monoclonal antibody (PICK1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag

   Plasmid

   #185682

   Purpose

   Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Human, Synthetic)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   K599A

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-Atoh1

   Plasmid

   #158529

   Purpose

   Expresses mouse Atoh1 in mammalian cells

   Depositor

   Neil Segil

   Article

   Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

   Insert

   Atonal BHLH Transcription Factor 1 (Atoh1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-EGFP-AC8(1-106)

   Plasmid

   #181958

   Purpose

   Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   EGFP-AC8(1-106) (Adcy8 Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CMV-Grid2(I677C)

   Plasmid

   #164782

   Purpose

   cysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligand

   Depositor

   Alexandre Mourot

   Article

   Lemoine et al Elife. 2020 Oct 28;9. pii: 59026. doi: 1

   Insert

   Grid2 I677C (Grid2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I677C

   Promoter

   CMV

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV superYFP-AURKA-mTurq2

   Plasmid

   #157772

   Purpose

   Expression of AuroraA kinase biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   mTurquoise2 and superYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Anti-Neuroligin-1 [N97A/31R]

    Plasmid

    #114554

    Purpose

    Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. kanMX6-ins6

    Plasmid

    #195043

    Purpose

    pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Venus-BimEL-pEGFP-C1

    Plasmid

    #166735

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    BimEL (Bcl2l11 Mouse)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123338

    Purpose

    Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    DAKAP-Venus-cpVenus-FLARE-AKAR
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1, Venus, and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE-80L 2xHis-LRRK2 Armadillo 1-552

    Plasmid

    #186017

    Purpose

    2xHis-LRRK2 Armadillo 1-552

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-LRRK2 Armadillo 1-552 (LRRK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag

    Plasmid

    #185681

    Purpose

    Low-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Human, Synthetic)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Deletion of lumenal domain

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flrt1-Fc-His

    Plasmid

    #72073

    Purpose

    Expresses the extracellular region of the FLRT1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Flrt1 (Flrt1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE2 mCherry-Rtn4HD

    Plasmid

    #86685

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4 homology domain (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    homology domain only

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-mCherry-KATNA1

    Plasmid

    #180650

    Purpose

    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GGACAGCACUCCCUUGAAA

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

    Plasmid

    #185684

    Purpose

    Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Human, Synthetic)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    K121Y

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE2 GFP-Rtn4HD

    Plasmid

    #86686

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4 homology domain (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    homology domain only

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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