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pmCellFree_KA1_ZIKV NS2A

Plasmid

#145343

Purpose

Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Feb. 20, 2021

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS7

Plasmid

#111761

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS7-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-G97

Plasmid

#130705

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS5

Plasmid

#145358

Purpose

Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Tags

mCherry and 8xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-PI(WT)-Vav2(DPZ)

Plasmid

#86974

Purpose

PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/C1 (Vav2 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-C-8His-Flag

Plasmid

#157678

Purpose

C-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

8His-Flag

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1P shTFRC

Plasmid

#169882

Purpose

Suppress TRFC

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

shRNA against TFRC

Use

Lentiviral and RNAi

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

Plasmid

#112170

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

mRuby3

Mutation

F102G

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2-hSyn1-NES-ChemoG-CaM

Plasmid

#193817

Purpose

Expression of ChemoG-CaM in primary neurons

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-CaM

Use

AAV

Mutation

EGFP:A206K; HT7: L271E

Promoter

hSyn1

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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miR-vector-RFP pCDNA

Plasmid

#97143

Purpose

for expression of miRNA and TagRFP

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Tags

TagRFP

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoL-ATP

Plasmid

#193831

Purpose

CMV overexpression of ChemoL-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-ATP

Expression

Mammalian

Mutation

EGFP:A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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RSRF-Luc-2mut

Plasmid

#31819

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Expression

Mammalian

Mutation

two CTATATTTAG RSRF sites mutated to CGATATTTCG

Promoter

mutated MEF2-dependent (-307 to -242 of Nur77/NR4…

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Muc2D1D2D3

Plasmid

#155215

Purpose

Expresses human MUC2 residues 21-1259

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3 (MUC2 Human)

Tags

6xHistadine

Expression

Mammalian

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR.R205A

Plasmid

#112162

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.R205A

Use

AAV

Mutation

R205A (no GABA binding)

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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GST-Cav FL (1-178)

Plasmid

#22445

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1(1-178)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Tab1-3'UTR

Plasmid

#97155

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Tab1 3'UTR

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKVC

Plasmid

#145339

Purpose

Cell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δnef/U3

Plasmid

#101346

Purpose

Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta nef/LTR

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pKPY197

Plasmid

#62599

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr412Gly-CePheRS Alpha Subunit

Tags

HIS

Expression

Worm

Mutation

Thr412Gly

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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