We narrowed to 10,252 results for: tre promoter

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pGEX-6P-1-DmGW182_862-1384-del1004-1115_L

Plasmid

#146872

Purpose

Bacterial Expression of DmGW182_862-1384-del1004-1115

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182_862-1384-del1004-1115 (gw Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-delCterm-delDUF-dsRNAres_L

Plasmid

#146827

Purpose

Insect Expression of DmGW182-delCterm-delDUF-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-delCterm-delDUF-dsRNAres (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-delUBAdelQrich_G

Plasmid

#146396

Purpose

Insect Expression of DmGW182-delUBAdelQrich

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182-delUBAdelQrich (gw Fly)

Expression

Insect

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-D-kcnj1a.1

Plasmid

#173125

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj1a (kcnj1a.1 Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR_Trf2

Plasmid

#125159

Purpose

Gateway entry clone

Depositor

Julius Brennecke, Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

Trf2 (Trf2 Fly)

Use

Gateway Cloning

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1-ATG7 3'UTR(406)

Plasmid

#122070

Purpose

Expressing ATG1 with ATG7 3'UTR

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native 3'UTR was replaced by ATG7 3'…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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isoform PKC Epsilon (JCE592)

Plasmid

#89776

Purpose

yeast expression of mouse PKC Epsilon with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC Epsilon (Prkce Mouse)

Expression

Mammalian

Promoter

yeast PKC1 promoter (-600 to -1)

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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CS2-mCHY-dASUN

Plasmid

#47042

Purpose

Fluorescent reported for dASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (asun Fly)

Tags

mCHY

Expression

Mammalian

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag

Plasmid

#179719

Purpose

Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag

Plasmid

#179723

Purpose

Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pIVTR(T7G)-NS1-TX91

Plasmid

#172289

Purpose

In vitro transcription of NS1 Influenza A virus protein (strain Texas91) for enhanced in vitro transcribed mRNA translation

Depositor

Patrik Verstreken

Insert

NS1-TX91

Use

Other

Promoter

T7 promoter G-initiating

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pIVTR(T7A)-Cas9-T2A-mClover3

Plasmid

#172293

Purpose

In vitro transcription of Cas9-T2A-mClover3 synthetic construct

Depositor

Patrik Verstreken

Insert

Cas9-T2A-mClover3

Use

CRISPR

Promoter

T7 promoter A-initiating

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pIVTR(T7A)-PuroR-T2A-mScarlet

Plasmid

#172301

Purpose

In vitro transcription of PuroR-T2A-mScarlet synthetic construct

Depositor

Patrik Verstreken

Insert

PuroR-T2A-mScarlet

Use

Other

Promoter

T7 promoter A-initiating

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pgRGFP

Plasmid

#82695

Purpose

Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

PGK, U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

Plasmid

#13617

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65, Thr 69 and Ser70 to Asp

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

Plasmid

#13616

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Ser 65, Thr 69 and Ser 70 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
iNos-eGFP

Plasmid

#207251

Purpose

Reporter for expression of eGFP under control of iNos promoter

Depositor

Michelle Farkas

Article

Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

Insert

iNos Promoter (Nos2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pACDura3

Plasmid

#138711

Purpose

Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Insert

ura3 gene (URA3 Budding Yeast)

Expression

Bacterial

Mutation

optimized ura3 gene for expression in Escherichia…

Promoter

pEM7 promoter

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-PPP2R5E siRNA-resistant

Plasmid

#120861

Purpose

expresses dox-inducible PPP2R5E resistant to siRNA pool 2 used in manuscript

Depositor

Emily Foley

Article

Lee et al J Cell Sci. 2017 May 15;130(10):1760-177

Insert

PPP2R5E (PPP2R5E Human)

Tags

FLAG

Expression

Mammalian

Mutation

silent mutation at aa 282-284 in PPP2RE to genera…

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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