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Showing: 2841 - 2860 of 2972 results

  6. pET11a-DAAT-V33G/T242G

    Plasmid
    #159278
    Purpose
    Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan
    Depositor
    Roberto Chica
    Publication
    Parmeggiani et al ACS Catalysis 9, 3482–3486.
    Insert
    Engineered d-alanine aminotransferase
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Valine 33 and threonine 242 have been mutated to …
    Promoter
    T7
    Available since
    Sept. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159278-plasmid-map-sequence-id-311390

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  7. pCDF-casBCDE(-6)

    Plasmid
    #89730
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Publication
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a shorter vers…
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89730-plasmid-map-sequence-id-177596

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  8. pCDF-casBCDE(-12)

    Plasmid
    #89731
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Publication
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a shorter vers…
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89731-plasmid-map-sequence-id-182925

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  9. pCDF-casBCDE(-18)

    Plasmid
    #89732
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Publication
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a shorter vers…
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89732-plasmid-map-sequence-id-177593

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  10. pCDF-casBCDE(-3)

    Plasmid
    #89729
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Publication
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a shorter vers…
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89729-plasmid-map-sequence-id-177590

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  11. pJK500

    Plasmid
    #72245
    Purpose
    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and endogenous
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72245-plasmid-map-sequence-id-140434

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  13. mutpRL-TK 6xUTR

    Plasmid
    #40761
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    6 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. mutpRL-TK 2xUTR

    Plasmid
    #40757
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    2 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. mutpRL-TK 4xUTR

    Plasmid
    #40759
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    4 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. mutpRL-TK 5xUTR

    Plasmid
    #40760
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    5 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. mutpRL-TK 1xUTR

    Plasmid
    #40756
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pJK048

    Plasmid
    #75230
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Publication
    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    75230-plasmid-map-sequence-id-142240

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  19. ATP11C-EQ-mCherry

    Plasmid
    #255862
    Purpose
    Expression of human ATP11C E184Q mutant, in mammalian cells, C-terminally tagged with mCherry
    Depositor
    Joseph Lyons
    Publication
    Scholtissek et al J Membrane Biol 259, 32 (2026)
    Insert
    ATP11C (ATP11C Human)
    Tags
    8xHis, Twin-Strep-tag, and mCherry with 3C cleava…
    Expression
    Mammalian
    Mutation
    changed Glutamate 184 to Glutamine
    Promoter
    CMV
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    255862-plasmid-map-sequence-id-515788

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Showing: 2841 - 2860 of 2972 results