Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 11,520 results for: Kars

Showing: 5721 - 5760 of 11520 results

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pAc5.1B-lambdaN-HA-DmNot1-Q1271A-dsRNAres_R

Plasmid

#147360

Purpose

Insect Expression of DmNot1-Q1271A-dsRNAres

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

DmNot1-Q1271A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-R1267A-dsRNAres_R

Plasmid

#147361

Purpose

Insect Expression of DmNot1-R1267A-dsRNAres

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

DmNot1-R1267A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsRCK_1-295_O

Plasmid

#147098

Purpose

Mammalian Expression of HsRck_1-295

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsRck_1-295 (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsRCK_296-472_O

Plasmid

#147099

Purpose

Mammalian Expression of HsRck_296-472

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsRck_296-472 (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GGSG_M

Plasmid

#146950

Purpose

Insect Expression of DmAGO1-del942-971-GGSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del942-971-GGSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GSG_M

Plasmid

#146951

Purpose

Insect Expression of DmAGO1-del942-971-GSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del942-971-GSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GGSG_M

Plasmid

#146948

Purpose

Insect Expression of DmAGO1-del835-886-GGSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del835-886-GGSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GSG_M

Plasmid

#146949

Purpose

Insect Expression of DmAGO1-del835-886-GSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del835-886-GSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1-1089_K

Plasmid

#146804

Purpose

Mammalian Expression of HsNot1iso1_1-1089

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1-1089 (CNOT1 Human)

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1085-1605_K

Plasmid

#146805

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot1iso1_1-1089_K

Plasmid

#146806

Purpose

Mammalian Expression of HsNot1iso1_1-1089

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1-1089 (CNOT1 Human)

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1085-1605_K

Plasmid

#146807

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH

Plasmid

#148878

Purpose

Mammalian Expression of TNFalpha-ARE-polyA-Malat

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

TNFalpha-ARE-polyA-Malat (TNF Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF2_35-54-GB1_AE

Plasmid

#148558

Purpose

Bacterial Expression of HsGIGYF2_35-54

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_35-54 (GIGYF2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF2_35-72-GB1_AE

Plasmid

#148559

Purpose

Bacterial Expression of HsGIGYF2_35-72

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_35-72 (GIGYF2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-HsGIGYF2_35-105-GB1_AE

Plasmid

#148560

Purpose

Bacterial Expression of HsGIGYF2_35-105

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_35-105 (GIGYF2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_1-234_AE

Plasmid

#148561

Purpose

Bacterial Expression of Hs4EHP_1-234

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234 (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-CvH-Hs4EHP_1-234-I85AM101A_AE

Plasmid

#148562

Purpose

Bacterial Expression of Hs4EHP_1-234-I85AM101A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234-I85AM101A (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-CvH-Hs4EHP_1-234-W95A_AE

Plasmid

#148563

Purpose

Bacterial Expression of Hs4EHP_1-234-W95A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234-W95A (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-CvH-Hs4EHP_52-234opt-R103LE149L_AE

Plasmid

#148565

Purpose

Bacterial Expression of Hs4EHP_52-234opt-R103LE149L

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt-R103LE149L (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpH-Hs4EHP_52-234opt-Q159SM161DR202E_AE

Plasmid

#148567

Purpose

Bacterial Expression of Hs4EHP_52-234opt-Q159SM161DR202E

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt-Q159SM161DR202E (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A-GB1_AE

Plasmid

#148556

Purpose

Bacterial Expression of HsGIGYF1_33-103-E44AE45FQ87A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-103-E44AE45FQ87A (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
DDX11-1

Plasmid

#155503

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX11 (DDX11 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-Hs4EHP-S99N_AF

Plasmid

#148692

Purpose

Mammalian Expression of Hs4EHP-S99N

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-S99N (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsGIGYF2_1-180-E88AN97F_AF

Plasmid

#148688

Purpose

Mammalian Expression of HsGIGYF2_1-180-E88AN97F

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-E88AN97F (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsGIGYF2_1-180-LFIENtoDDDAF_AF

Plasmid

#148689

Purpose

Mammalian Expression of HsGIGYF2_1-180-LFIENtoDDDAF

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-LFIENtoDDDAF (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsGIGYF2_1-180-LFIPLtoDDDDA_AF

Plasmid

#148690

Purpose

Mammalian Expression of HsGIGYF2_1-180-LFIPLtoDDDDA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-LFIPLtoDDDDA (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsGIGYF2_1-180-P78DL79A_AE

Plasmid

#148579

Purpose

Mammalian Expression of HsGIGYF2_1-180-P78DL79A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-P78DL79A (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsGIGYF2_1-180-YYMLtoAAAA_AE

Plasmid

#148580

Purpose

Mammalian Expression of HsGIGYF2_1-180-YYMLtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-YYMLtoAAAA (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-HsGIGYF2_1-180-LFItoDDD_AE

Plasmid

#148582

Purpose

Mammalian Expression of HsGIGYF2_1-180-LFItoDDD

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-LFItoDDD (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-Hs4EHP-I85AM101A_AE

Plasmid

#148583

Purpose

Mammalian Expression of Hs4EHP-I85AM101A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-I85AM101A (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-Hs4EHP-I85AW95AM101A_AE

Plasmid

#148584

Purpose

Mammalian Expression of Hs4EHP-I85AW95AM101A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-I85AW95AM101A (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-Hs4EHP-R103LE149L_AE

Plasmid

#148585

Purpose

Mammalian Expression of Hs4EHP-R103LE149L

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-R103LE149L (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-Hs4EHP-W95AR103LE149L_AE

Plasmid

#148587

Purpose

Mammalian Expression of Hs4EHP-W95AR103LE149L

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-W95AR103LE149L (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot9-shRNAres1_W

Plasmid

#147904

Purpose

Mammalian Expression of HsNot9-shRNAres1

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-shRNAres1 (CNOT9 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KCC2 scFv [N1/12]

Plasmid

#190520

Purpose

Mammalian Expression of KCC2 scFV. Derived from hybridoma N1/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

KCC2 (Rattus norvegicus) recombinant scFV (Slc12a5 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CSTF3

Plasmid

#155487

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CSTF3 (CSTF3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST2-GST-TEV-OPTN S177D S473D

Plasmid

#187827

Purpose

Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

OPTN (OPTN Human)

Tags

GST-TEV

Expression

Bacterial

Mutation

S177D S473D

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a Sa-iPGM-bio

Plasmid

#179523

Purpose

Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

S. aureus iPGM bio-6His

Tags

His tag and biotinylation sequence

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a Mo-iPGM-bio

Plasmid

#179614

Purpose

Expresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

M. orale iPGM bio-6His

Tags

His tag and biotinylation sequence

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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