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AGG1829 OpIE2-Bn

Plasmid

#242438

Purpose

Insect OpIE2 promoter expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

Barnase (J5X95_RS06160 Bacillus amyloliquefaciens)

Expression

Insect

Promoter

OpIE2

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1012

Plasmid

#239934

Purpose

Csy4 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Csy4

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1021

Plasmid

#239943

Purpose

Renilla luciferase as a Level-0 part (Golden Gate)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Rluc

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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HSA_3b*_AP

Plasmid

#238899

Purpose

Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

Recombinant Human Serum Albumin (HSA) 3b*

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_TEV_aMFx1 (4a)

Plasmid

#238903

Purpose

Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_TEV_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_C-myc (4a)

Plasmid

#238907

Purpose

Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_C-myc

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-Vector

Plasmid

#239611

Purpose

Transposon vector

Depositor

Xin-yuan Guan

Article

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-SAP30

Plasmid

#109212

Purpose

Encodes human full-length SAP30 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

Human full-length SAP30 (SAP30 Human)

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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YEE_GFP

Plasmid

#228184

Purpose

Yeast episomal expression vector with type '234' GFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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YEE_RFP

Plasmid

#228185

Purpose

Yeast episomal expression vector with type '234' RFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHR TRE_SEAP_IRES_mCherry-PGK_BFP-hf

Plasmid

#223535

Purpose

Inducible secreted embryonic alkaline phosphatase and mCherry; constitutive BFP

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Inserts

Secreted alkaline phosphatase
mCherry
TagBFP

Use

Lentiviral

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

Plasmid

#233067

Purpose

Piggybac CRISPRi construct

Depositor

Markus Landthaler

Article

Vučićević et al bioRxiv 2024.09.12.612198

Insert

KRAB-dCas9

Use

CRISPR

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINS-HOIP NZF1 (351-379)

Plasmid

#235655

Purpose

Bacterial expression of human HOIP NZF1 (351-379)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

HOIP NZF1 (RNF31 Human)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 351-379 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-ZRANB3 NZF (619-650)

Plasmid

#235654

Purpose

Bacterial expression of human ZRANB3 NZF (619-650)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

ZRANB3 NZF (ZRANB3 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 619-650 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-Sharpin NZF (347-376)

Plasmid

#235651

Purpose

Bacterial expression of human Sharpin NZF (347-376)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

Sharpin NZF (SHARPIN Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 347-376 only

Promoter

T7

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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Strep-Tev-Avi-hZO-1

Plasmid

#236527

Purpose

For expression of ZO-1 (human) in insect cells

Depositor

Sandra Citi

Article

Spadaro et al Curr Biol. 2017 Dec 18;27(24):3783-3795.

Insert

TJP1 (TJP1 Human)

Tags

SPY Tag and StrepTag-Tev-AviTag

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-E1GFP

Plasmid

#233885

Purpose

Bacterial Expression of recombinant His-tagged E1GFP

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

E1GFP

Tags

His

Expression

Bacterial

Mutation

T65S and T203Y compared to EGFP

Promoter

T7 promoter

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0140 CMVTO-Renin-ECL-CD4-tevs-RXR6.2

Plasmid

#231199

Purpose

ER-retained renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin-ECL-CD4-tevs-RXR6.2 (REN Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0151 CMVTO-Renin(H2M)-ECL

Plasmid

#231201

Purpose

Membrane-bound human-to-mouse (H2M) renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(H2M)-ECL (REN Human)

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CMVTO-hIL2 (AV)

Plasmid

#231226

Purpose

hIL2

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL2 (IL2 Human)

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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