We narrowed to 4,430 results for: TIL;

1. pFA6a-kanMX6-P81nmt1

   Plasmid

   #39282

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Mutation

   -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

   Available Since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC-PreE2

   Plasmid

   #52264

   Purpose

   Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   Ubc9 GST fusion (UBE2I Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TS MS-Sensor

   Plasmid

   #199788

   Purpose

   Synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

   Insert

   TS MS-Sensor
   

   Use

   Synthetic Biology

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCB15

   Plasmid

   #194756

   Purpose

   GluRIIA-Rescue-Construct for integration at attP sites

   Depositor

   Sven Dannhäuser

   Article

   Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

   Insert

   GluRIIA (GluRIIA Fly)
   

   Use

   Phic31-integration vector

   Mutation

   none

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SORT1-bio-His

   Plasmid

   #52024

   Purpose

   Expresses full-length Sortilin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SORT1 (SORT1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

   Plasmid

   #169548

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDL14

   Plasmid

   #106901

   Purpose

   pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Inserts

   gam
   bet
   mutS
   lacI
   rhaR
   rhaS
   

   Expression

   Bacterial

   Mutation

   Lysine 622 was replaced by Alanine

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6-CjCas9-MS2gRNA-Backbone

   Plasmid

   #210711

   Purpose

   This plasmid express MS2 loop containing Cj Cas9 gRNA

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MS2 loop containing Cj Cas9 gRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. JN302: pMVP/SB/Puro-DEST

   Plasmid

   #121865

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVPT-GDNF-tTR-KRAB

    Plasmid

    #11646

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for GDNF (mPGK promoter) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GDNF, tTR-KRAB, Tet-on (GDNF Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-CRY2-mCherry-MYPT169

    Plasmid

    #178526

    Purpose

    Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CRY2-mCherry-MYPT169
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-kanMX6-P41nmt-3HA

    Plasmid

    #39284

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Mutation

    -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

    Available Since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pYPQ-SpRY-Act3.0

    Plasmid

    #178957

    Purpose

    It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TT MS-Sensor

    Plasmid

    #199789

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    TT MS-Sensor
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-kanMX6-P81nmt1-3HA

    Plasmid

    #39285

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Mutation

    -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

    Available Since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pSK+ chickBMPR-1B DN

    Plasmid

    #49529

    Purpose

    cloning vector for dominant negative chick BMP receptor 1B

    Depositor

    Lee Niswander, Peter ten Dijke

    Article

    Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

    Insert

    BMPR-1B DN (BMPR1B Chicken)
    

    Expression

    Bacterial

    Mutation

    dominant negative mutation (K231R); also P2A, M11…

    Promoter

    T3

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

17. PTPRA_deltaD1-SHC

    Plasmid

    #139615

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    PTPRA_deltaD1 (PTPRA Human)
    

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Mutation

    D1 domain deletion

    Promoter

    CMV

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSP1594_2x_NLS

    Plasmid

    #110625

    Purpose

    Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    None
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSC-IntE2

    Plasmid

    #52265

    Purpose

    Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    Ubc9 GST fusion (UBE2I Human, Synthetic)
    

    Tags

    GST and GyrA-intein

    Expression

    Bacterial

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. GIT1A-FLAG

    Plasmid

    #15225

    Depositor

    Rick Horwitz

    Article

    Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

    Insert

    G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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