We narrowed to 3,194 results for: pEGFP

1. HA-BAP1-C91W-GFP

   Plasmid

   #78913

   Purpose

   Mammalian expression of BAP1 with C91W and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

   Insert

   BAP1 (BAP1 Human)
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   C91W

   Promoter

   CMV

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pKIF1C-BioID2-HA

   Plasmid

   #130980

   Purpose

   Expression of HA-tagged KIF1C-BioID2

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   KIF1C-BioID2-HA (KIF1C Human)
   

   Tags

   BioID2-HA

   Expression

   Mammalian

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   CMV

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD8a-hnRNP-M-EGFP

   Plasmid

   #86054

   Purpose

   A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   CD8a (CD8A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CD8a luminal and transmembrane domians along with…

   Promoter

   CMV

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-CRY2-P14P5K

   Plasmid

   #79569

   Purpose

   expression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

   Depositor

   Olof Idevall-Hagren

   Article

   Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

   Insert

   Pip5k1c (Pip5k1c Mouse)
   

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   R445E and R446E; truncation of amino acids 636-66…

   Promoter

   CMV

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

5. GFP-AuroraB L154G/H250Y

   Plasmid

   #108492

   Purpose

   expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Analog sensitive L154G/H250Y

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-BAP1-N102S-GFP

   Plasmid

   #78915

   Purpose

   Mammalian expression of BAP1 with N102S and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

   Insert

   BAP1 (BAP1 Human)
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   N102S

   Promoter

   CMV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCIBN-tagBFP-hNCL

   Plasmid

   #103805

   Purpose

   BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIBN-LacI

   Plasmid

   #103814

   Purpose

   BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-LacI (CIB1, lacI Mustard Weed, E. coli)
   

   Expression

   Mammalian

   Mutation

   LacI: C19T (silent, L7L), T264C (silent, A88A), C…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pN1 VAMP2 R125TAG

   Plasmid

   #69877

   Purpose

   express in mammalian cells for unnatural amino acid incorporation into VAMP2

   Depositor

   Silvio Rizzoli

   Article

   Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   Amber and Ochre stop codons in the former linker …

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCIBN-TetR-tagRFP-T

    Plasmid

    #103809

    Purpose

    'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)
    

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIBN-EYFP-hNCL

    Plasmid

    #103806

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hNCL (CIB1, NCL Human, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. FKBP-beta2(616-951)-GFP Double

    Plasmid

    #100729

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform, deletion of clathrin box motif and …

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE4561

    Plasmid

    #107525

    Purpose

    Expresses Lb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    mCherry
    

    Expression

    Mammalian

    Promoter

    CBh and human U6

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. FKBP-beta2(616-951)-GFP ∆CBM

    Plasmid

    #100728

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform, deletion of clathrin box motif

    Promoter

    CMV

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. CD8a-cNLS-EGFP

    Plasmid

    #86052

    Purpose

    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CD8a luminal and transmembrane along with two SV4…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-RanV19 E1RA2

    Plasmid

    #118317

    Purpose

    Expression of human Ran

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Inserts

    RanV19 (RAN Human)
    EPac1RA2 (RAPGEF3, RAPGEF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    in-frame insertion of RanV19 into GFPEpac1RA2 and…

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Drosha270-1374S300/302A

    Plasmid

    #62533

    Purpose

    N-terminal aa1-269 of Drosha is deleted and serines 300 &302 are mutated to alanine

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    truncated human Drosha mutant (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-269 and changed serines 300…

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. GFP-Drosha270-1374delNLS2

    Plasmid

    #62532

    Purpose

    N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    truncated human Drosha mutant (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-269 and nuclear localizatio…

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

19. HLH Clock Pas 1-401 VenN

    Plasmid

    #47353

    Purpose

    Clock fragment cloned and tagged with VenN

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401

    Promoter

    CMV

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

20. HLH Bmal Pas 1-465-VenC I317D

    Plasmid

    #47367

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, I317D

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only