We narrowed to 17,207 results for: puro

1. pEpicVector3mC_Puro-T2A-mCherry-ProtC-FLAG-AU1

   Plasmid

   #162117

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to ProtC-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   ProtC-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEpicVector3mC_Puro-T2A-mCherry-VSVg-FLAG-AU1

   Plasmid

   #162108

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-FLAG

   Plasmid

   #162084

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   VSVg-ProtC-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-AU1

   Plasmid

   #162091

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-ProtC-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti spCas9 T2A mNeonGreen P2A puro

   Plasmid

   #122183

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9 (NEWENTRY )
   mNeonGreen
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

   Plasmid

   #134661

   Purpose

   Expresses EGFP-tagged synaptic vesicle protein

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   vesicle-associated membrane protein 2 (VAMP2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

   Plasmid

   #194925

   Purpose

   mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

   Depositor

   Hao Xu

   Article

   Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHage2-EF1a-TERT K78E-IRES-Puro

   Plasmid

   #182591

   Purpose

   expresses TERT K78E in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   K78E

   Promoter

   EF-1a

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

   Plasmid

   #193465

   Purpose

   Dn29 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti CMV Puro DEST (w118-1) SDC3

    Plasmid

    #107501

    Purpose

    Expresses SDC3, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    SDC3 (codon optimized) (SDC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

    Plasmid

    #134865

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Membrin (GOSR2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-EZH2-PGK-Puro-IRES-GFP

    Plasmid

    #75125

    Purpose

    Retroviral expression plasmid encoding wild type human EZH2

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    KMT6 (EZH2 Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)

    Plasmid

    #208395

    Purpose

    To generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    DB1delta/DB2delta

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PLKO-mcherry-luc-puro-miR-211

    Plasmid

    #29784

    Depositor

    Carl Novina

    Article

    Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

    Insert

    miR-211
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Use

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid

    #117134

    Purpose

    All-in-one expression vector for Cas9 and gRNA against CD44

    Depositor

    Kosuke Yusa

    Insert

    gCD44 v2 (Cd44 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. delta PE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52380

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -1620 - -1011 upstream from OCT4 ATG

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2T-CAG-REV1-p2A-GFP-PuroR

    Plasmid

    #153001

    Purpose

    Overexpression of human REV1

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    REV1 (REV1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid

    #122200

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9
    TagRFP-T
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. delta DE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52381

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -3194 - -1768 upstream from OCT4 ATG

    Promoter

    OCT4

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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