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We narrowed to 1,582 results for: Mbp

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Showing: 1441 - 1450 of 1582 results

  1. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid
    #179527
    Purpose
    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 K233A/K234A/K253A/K263A
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed lysines 233, 234, 253, and 263 to alanine
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179527-plasmid-map-sequence-id-351733

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  10. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Plasmid
    #101214
    Purpose
    Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/K855A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C and K855A
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101214-plasmid-map-sequence-id-197223

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Showing: 1441 - 1450 of 1582 results