We narrowed to 5,122 results for: NOG

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pRSFDuetG-DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127_P

Plasmid

#147220

Purpose

Bacterial Expression of DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127 (pcm Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA2

Plasmid

#186664

Purpose

Nbr C-tag sgRNA2 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 2 Plasmid (Nbr Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA1

Plasmid

#186663

Purpose

Nbr C-tag sgRNA1 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 1 Plasmid (Nbr Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Shu_sgRNA

Plasmid

#186656

Purpose

shu sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Shu sgRNA Plasmid (shu Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_nonPuro_OSS

Plasmid

#186652

Purpose

Cas9 plasmid to express the Cas9 in OSS cells

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Cas9 Expression Plasmid

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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VIR_sgRNA

Plasmid

#186661

Purpose

VIR sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

VIR sgRNA Plasmid (vir Fly)

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAT079

Plasmid

#180509

Purpose

Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
DpbCasX

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P

Plasmid

#147209

Purpose

Insect Expression of DmXRN1-P1339AP1342A-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1-P1339AP1342A-dsRNAres (pcm Fly)

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-FLAG-CBPΔ(N+HAT)

Plasmid

#182841

Purpose

An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.

Depositor

Peter Harte

Article

Tie et al Development. 2009 Sep;136(18):3131-41. d

Insert

CBP (w Fly)

Tags

FLAG tag

Expression

Insect

Mutation

dletion of residues 1566-2142 from CBP1476-3222

Promoter

MT

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_163 SpCas9 K233A/K234A/K253A/K263A

Plasmid

#179527

Purpose

For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 K233A/K234A/K253A/K263A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed lysines 233, 234, 253, and 263 to alanine

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam_141-442_AG

Plasmid

#148833

Purpose

Bacterial Expression of DmBam_141-422

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_141-422 (bam Fly)

Expression

Bacterial

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC

Plasmid

#148402

Purpose

Insect Expression of DmNot1_1674-1962-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1_1674-1962-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMextli-M605D_AB

Plasmid

#148344

Purpose

Insect Expression of DmMextli-M605D

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli-M605D (mxt Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R

Plasmid

#147433

Purpose

Insect Expression of DmNot1_1711-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1711-2505-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmMextli_577-620-I583RS590R-GB1_AB

Plasmid

#148357

Purpose

Bacterial Expression of DmMextli_577-620-I583RS590R

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-620-I583RS590R (mxt Fly)

Expression

Bacterial

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-R56ER63E-GB1_AA

Plasmid

#148272

Purpose

Bacterial Expression of DmThor_50-83-R56ER63E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_50-83-R56ER63E (Thor Fly)

Expression

Bacterial

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1148_I

Plasmid

#146604

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Expression

Insect

Mutation

4 silent and two non silent N605S, K759M mutation…

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSmTex-del46-49-V5His6_B

Plasmid

#145995

Purpose

Insect Expression of DmTral-LSmTex-del46-49

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmTex-del46-49 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSmTex-del46-49-V5His6_B

Plasmid

#145985

Purpose

Insect Expression of DmTral-LSmTex-del46-49

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmTex-del46-49 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D

Plasmid

#146129

Purpose

Insect Expression of DmEDC3_102-330

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3_102-330 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSm-del46-60-V5His6_C

Plasmid

#146068

Purpose

Insect Expression of DmTral-LSm-del46-60

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSm-del46-60 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C

Plasmid

#146046

Purpose

Insect Expression of DmTral-LSm-del46-60

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSm-del46-60 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_A

Plasmid

#145894

Purpose

Insect Expression of DmCCR4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmCCR4 (twin Fly)

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG1856_A

Plasmid

#145905

Purpose

Insect Expression of CG1856-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jan . 15(1):21-32.

Insert

CG1856-3UTR (ttk Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

Plasmid

#146957

Purpose

Insect Expression of DmAGO1-K690A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K690A (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-EnR-MCS_N-term

Plasmid

#136884

Purpose

Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-EnR_C-term

Plasmid

#136885

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 TNos:Luc:5'UTR (GB1499)

Plasmid

#160580

Purpose

Reversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoter

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

Luc

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 integrase (GB1561)

Plasmid

#160586

Purpose

Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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