We narrowed to 9,185 results for: Pol;

1. pMXs-PT2A-GFP-iRFP670-Tdtomato

   Plasmid

   #111813

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   GFP
   iRFP670
   Tdtomato
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-PT2A-iRFP670-GFP-Tdtomato

   Plasmid

   #111816

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   iRFP670
   GFP
   Tdtomato
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pREF-GFP kan

   Plasmid

   #122061

   Purpose

   reporter plasmid for termination and terminator override to express GFP. Marked with URA3

   Depositor

   Daniel Reines

   Article

   Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

   Inserts

   intergenic terminator from yeast IMD2
   green fluorescent protein
   

   Expression

   Yeast

   Promoter

   GAL1 and GAL1 comes in pYES2

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21(+)-His6-Ubc13

   Plasmid

   #212715

   Purpose

   Bacterial expression of His6-Ubc13

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Ubc13
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-Muc2D1D2D3

   Plasmid

   #155215

   Purpose

   Expresses human MUC2 residues 21-1259

   Depositor

   Deborah Fass

   Article

   Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

   Insert

   Muc2 D1D2D3 (MUC2 Human)
   

   Tags

   6xHistadine

   Expression

   Mammalian

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. NylB_pET29b

   Plasmid

   #215414

   Purpose

   Expression construct for NylB gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB
   

   Expression

   Bacterial

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p426 25Q GAL

   Plasmid

   #1187

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pBTRCk-pVhb-maACR

   Plasmid

   #215651

   Purpose

   pBBR1 Origin, KanR, with maACR gene under Vhb promoter

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Insert

   acyl-CoA reductase
   

   Tags

   Maltose binding protein and His tag

   Expression

   Bacterial

   Promoter

   Vhb

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYC-pVhb-vfFadBA

   Plasmid

   #215650

   Purpose

   pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Insert

   thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
   

   Expression

   Bacterial

   Promoter

   Vhb

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p426 72Q GPD

    Plasmid

    #1183

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 72Q (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pET21a - SodA

    Plasmid

    #89612

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase A
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

    Plasmid

    #172225

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCaUra3-CUbo-AID*-GFP

    Plasmid

    #212775

    Purpose

    C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAG-mCherry-Otx2shRNA1

    Plasmid

    #73979

    Purpose

    Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Otx2 gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET:GACT:GFP

    Plasmid

    #60734

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myc tag S16-A PTB

    Plasmid

    #23025

    Depositor

    Douglas Black

    Article

    Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

    Insert

    PTB1 (PTBP1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed Serine 16 to Alanine

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

17. GFAP-Archon-EGFP

    Plasmid

    #187979

    Purpose

    Expresses the fluorescent voltage indicator Archon under the GFAP promoter

    Depositor

    Chris Dulla

    Article

    Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

    Insert

    Archon1-EGFP
    

    Use

    AAV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-EnR

    Plasmid

    #19715

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    engrailed (en Fly)
    

    Expression

    Mammalian

    Mutation

    Drosophila engrailed repressor domain

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-hSyn-ConVERGD-ArchT(eGFP)-WPRE

    Plasmid

    #218760

    Purpose

    Provides AND intersectional (Cre+Flp) expression of ArchT-eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    ArchT
    

    Use

    AAV

    Tags

    eGFP

    Promoter

    hSyn

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB80-2xFKBP-L-mCherry-CAAX

    Plasmid

    #193713

    Purpose

    Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    2xFKBP-L-mCherry-CAAX
    

    Tags

    2xFKBP-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. AL13Rb-GAPC2

    Plasmid

    #161009

    Purpose

    PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Expression

    Plant

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. rat APOBEC1

    Plasmid

    #112858

    Purpose

    plasmid for expression of rat APOBEC1 in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204798

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pLMB757

    Plasmid

    #83792

    Purpose

    Fructose biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL0489 (frcB) containing all of RL0488 (frcR)
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pFastBac flag Bmi1

    Plasmid

    #1967

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pSicoR-mCh-Oct4i

    Plasmid

    #21906

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Insert

    Oct4i
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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27. Tt-NylC_pET28a

    Plasmid

    #215431

    Purpose

    Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Tt-NylC
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLMB713

    Plasmid

    #83785

    Purpose

    Salicylic acid biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL1329 (salA) containing all of RL1328 (salR)
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEMT-PT2A-Tdtomato-iRFP670-GFP

    Plasmid

    #111815

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Tdtomato
    iRFP670
    GFP
    

    Use

    Cloning intermediate

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCellFree_G03 RUVBL1

    Plasmid

    #67091

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RUVBL1 (RUVBL1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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