We narrowed to 9,353 results for: Uty

1. AAV-H2GH

   Plasmid

   #182202

   Purpose

   Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

   Depositor

   Joshua Kritzer

   Article

   Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

   Insert

   histone2B-HaloTag-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mOtop2_pcDNA3

   Plasmid

   #114678

   Purpose

   This construct was used to express mouse Otop2 in HEK-293 cells

   Depositor

   Emily Liman

   Article

   Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

   Insert

   Otopetrin 2 (Otop2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSETb-rsGreen0.7

   Plasmid

   #78176

   Purpose

   Expresses rsGreen0.7 in E. coli strains.

   Depositor

   Peter Dedecker

   Article

   Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

   Insert

   rsGreen0.7
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. BbZIP-cALFA

   Plasmid

   #160498

   Purpose

   For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   BbZIP
   

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-SIRT6-H133Y

   Plasmid

   #102327

   Purpose

   Human SIRT6 H133Y for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   changed histidine 133 to tyrosine

   Promoter

   CMV

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. APT3-VAMP4

   Plasmid

   #168004

   Purpose

   positive selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   VAMP4(I75-L91)-T3'PAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. APT3-PTEN (PTEN high)

   Plasmid

   #168006

   Purpose

   positive selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   PTEN-T3'PAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. untargeted Plk1 FRET sensor Myt1 substrate

   Plasmid

   #45217

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   untargeted Plk1 FRET sensor Myt1 substrate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03

   Plasmid

   #158793

   Purpose

   Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

   Insert

   Neuraminidase A/NL/109/03
   

   Use

   Tags

   TwinStrep_sfGFP_tetrabrachion

   Expression

   Mammalian

   Mutation

   AA61-469

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMART-GFPuv

    Plasmid

    #65822

    Purpose

    GFPuv Expression, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    gfpuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli insulated waaH gene promoter, low phosp…

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7RNAP Cyslight V174C

    Plasmid

    #205078

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V174C

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRXG

    Plasmid

    #113643

    Purpose

    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    mRFP1-linker-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3 Ras-LOCKR-S R89L (negative control)

    Plasmid

    #216497

    Purpose

    FRET-based reporter for Ras activity, untargeted, negative control

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S R89L negative control
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS-393

    Plasmid

    #195473

    Purpose

    Lentiviral Integratable Inducible super-IL2 cytokine + synZiFTR10

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF10 BS - ybTATA - IL12] - pSFFV - ZF10 - NS3 - p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4

    Plasmid

    #135460

    Purpose

    Mammalian expression of uFLARE TF component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
    

    Use

    Tags

    HA, flag,

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-CV-Ykt6

    Plasmid

    #168030

    Purpose

    Expresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-Ykt6(Q131-K190)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT29-splitD

    Plasmid

    #122599

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM1-Clover-Prx2-mRuby2

    Plasmid

    #117907

    Purpose

    fluorescent reporter of cytosolic hydrogen peroxide

    Depositor

    Hadley Sikes

    Article

    Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

    Insert

    Clover-Prx2-mRuby2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. T5-sgRNA

    Plasmid

    #113655

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter T5 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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