Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 62,126 results for: cloning

Clear keywords
Showing: 2881 - 2900 of 62126 results

  2. pSBbi-BH

    Plasmid
    #60515
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60515-plasmid-map-sequence-id-97531

    Enlarge

  5. pcDNA-3-VEGFR3

    Plasmid
    #119230
    Purpose
    Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.
    Depositor
    Kari Alitalo
    Insert
    VEGFR3 (FLT4 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119230-plasmid-map-sequence-id-231667

    Enlarge

  12. R777-E140 Hs.NF1-nostop

    Plasmid
    #70424
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70424-plasmid-map-sequence-id-125873

    Enlarge

  16. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid
    #49235
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49235-plasmid-map-sequence-id-376544

    Enlarge

  18. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. R777-E173 Hs.PIK3R2

    Plasmid
    #70457
    Purpose
    Gateway ORF clone of human PIK3R2 [NM_005027.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R2 (PIK3R2 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70457-plasmid-map-sequence-id-125906

    Enlarge

Showing: 2881 - 2900 of 62126 results