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We narrowed to 60,661 results for: cloning

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Showing: 2881 - 2900 of 60661 results

  1. PP-AK

    Plasmid
    #25119
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd5_3360:MAC029-C04:C34239 (cgd5_3360 Cryptosporidium parvum)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. PP-CyP5

    Plasmid
    #25121
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    49.m03261:C37453
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. PP-UBC13 (3E95)

    Plasmid
    #25131
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFE1350c:C34878
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. PP-GNPNAT1

    Plasmid
    #25281
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb11.01.2886:V5-L147
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  14. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  16. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Expression
    Mammalian
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

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  18. p3E-MCS

    Plasmid
    #75174
    Purpose
    Multiple cloning site for insertion of gene of interest for C-terminal fusions.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    Multiple Cloning Site
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75174-plasmid-map-sequence-id-142182

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  20. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

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Showing: 2881 - 2900 of 60661 results