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pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

Plasmid

#127792

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg3…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET-SOMA-T679A

Plasmid

#118936

Purpose

Used to express the T679A mutant form of the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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1476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spA

Plasmid

#109315

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Insert

Mttp gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC176

Plasmid

#118891

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6-MBP, cleavable with 3C protease; SNAP, cleav…

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-HA-TSAd-Y89XF

Plasmid

#84559

Purpose

mammalian expression of TSAd-Y89XF

Depositor

Anne Spurkland

Article

Granum et al J Biol Chem. 2008 Aug 8;283(32):21909-19

Insert

TSAd (SH2D2A Human)

Tags

HA

Expression

Mammalian

Mutation

Y279F, Y290F, Y305F

Promoter

EF1a

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
tdminiSOG-Tubulin-6

Plasmid

#57808

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PA-mCherry-miniSOG-Vinculin-23

Plasmid

#57801

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vinculin (VCL Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
miniSOG-Vinculin-23

Plasmid

#57780

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vinculin (VCL Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
DIRAS2

Plasmid

#55670

Purpose

expression of mouse DIRAS2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

DIRAS2 (Diras2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pFM038

Plasmid

#40141

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R183-5A + 7SA; base pairs 6-523 of par-2 were re…

Promoter

pie-1

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM041

Plasmid

#40144

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (S241A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM039

Plasmid

#40142

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R183-5A + S241A; base pairs 6-523 of par-2 were …

Promoter

pie-1

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM036

Plasmid

#40139

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (K162A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM052

Plasmid

#40146

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (C56S) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

C56S; base pairs 6-523 of par-2 were recoded to …

Promoter

pie-1

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pFM037

Plasmid

#40140

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R163A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R163A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
CS2 luc MutA

Plasmid

#13498

Depositor

Stephen Tapscott

Article

Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

Insert

Fstl1 (Fstl1 Mouse)

Use

Luciferase

Mutation

contains 3 tandem copies of the following sequenc…

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
CS2 luc MutB

Plasmid

#13499

Depositor

Stephen Tapscott

Article

Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

Insert

Fstl1 (Fstl1 Mouse)

Use

Luciferase

Mutation

contains 3 tandem copies of the following sequenc…

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

Plasmid

#115141

Purpose

CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Orf2mor anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-NRG1B1

Plasmid

#120287

Purpose

Produce human recombinant NRG1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

NRG1-beta 1

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-Itprip-V5

Plasmid

#120241

Purpose

Expresses V5-tagged Itprip in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Itprip (ITPRIP Human)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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