Addgene

We narrowed to 20,931 results for: Tes

Showing: 4351 - 4380 of 20931 results

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pCDNA4/TO-ORF68 (tagless)

Plasmid

#166025

Purpose

Expresses ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-EnRRbpj

Plasmid

#160925

Purpose

dominant-negative construct to inhibit Notch signaling

Depositor

Mark Emerson

Article

Chen et al Sci Rep. 2021 Jul 15;11(1):14525. doi: 1

Insert

EnRRbpj

Use

Tags

Expression

Mutation

fused with an engrailed repressor

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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p8208 MSCV-Neo C-HA EMPTY

Plasmid

#163310

Purpose

Empty vector control

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7-Rictor-shRNA

Plasmid

#157930

Purpose

Knockdown of Rictor

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Rictor shRNA

Use

Lentiviral and RNAi

Tags

tdTomato

Expression

Mutation

Promoter

mouse U6

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pre-Ubc4 BrG pUC19

Plasmid

#111322

Purpose

for transcription to Ubc4 pre-mRNA

Depositor

Christine Guthrie

Article

Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

Insert

UBC4 (UBC4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

intron has branch-site A to G mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dATG1 (1188)

Plasmid

#66918

Purpose

bacterial expression of dATG1

Depositor

Jun Hee Lee, Michael Karin

Article

Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

Insert

ATG1

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAP757-1

Plasmid

#99493

Purpose

6XHis::TEV::3XFlag::linker::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

6XHis::TEV::3XFlag::linker::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP605-1

Plasmid

#99482

Purpose

Myc::TEV::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::TEV::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP640-2

Plasmid

#99487

Purpose

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM30C

Plasmid

#87083

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30C (Trim30c Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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miniUAA5

Plasmid

#78484

Purpose

1336nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail

Depositor

Allan Jacobson

Article

Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d

Insert

CAN1-LUC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only
miniUAA6

Plasmid

#78485

Purpose

1559nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail

Depositor

Allan Jacobson

Article

Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d

Insert

CAN1-LUC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only
miniUAA4

Plasmid

#78483

Purpose

1105nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail

Depositor

Allan Jacobson

Article

Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d

Insert

CAN1-LUC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only
MetNI (E166Q in MetN)

Plasmid

#78320

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Johnson et al Protein Sci. 2012 Jan;21(1):84-96. doi:

Inserts

MetI
MetN

Use

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Mutation

E166Q

Promoter

T7 Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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miniUAA2

Plasmid

#78481

Purpose

600nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail

Depositor

Allan Jacobson

Article

Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d

Insert

CAN1-LUC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only
miniUAA3

Plasmid

#78482

Purpose

882nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail

Depositor

Allan Jacobson

Article

Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d

Insert

CAN1-LUC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only
pPB-CAG-aVent1-pgk-hph

Plasmid

#74916

Purpose

Mammalian expresion of aVent1

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Vent1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-aBsx-pgk-hph

Plasmid

#74913

Purpose

Mammalian expression of aBsx

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Bsx

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pGC461

Plasmid

#71363

Purpose

distal tip cell expression of DAF-16 in C. elegans

Depositor

Jane Hubbard

Article

Michaelson et al Development. 2010 Feb;137(4):671-80. doi

Insert

daf-16 (daf-16 Nematode)

Use

Tags

GFP(65C)

Expression

Worm

Mutation

Promoter

Blelloch et al., 1999; Mathies et al., 2003

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-ceRab2(1-214)

Plasmid

#59550

Purpose

makes 6x Fusion of Full length ceRab2

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-214

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab5(1-208)

Plasmid

#59551

Purpose

makes 6x Fusion of Full length ceRab5

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-208

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab10(1-201)

Plasmid

#59552

Purpose

makes 6x Fusion of Full length ceRab10

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-208

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab11(1-211)

Plasmid

#59553

Purpose

makes 6x Fusion of Full length ceRab11

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-211

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab21(1-207)

Plasmid

#59554

Purpose

makes 6x Fusion of Full length ceRab21

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-207

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET28-ceRab21(1-209)

Plasmid

#59555

Purpose

makes 6x Fusion of Full length ceRab35

Depositor

David Lambright

Article

Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

Insert

1-209

Use

Tags

6x His

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pMal mPtch-Cterm

Plasmid

#58705

Purpose

used for anti-mouse Patched antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Ptch (Ptch1 Mouse)

Use

Tags

MBP

Expression

Bacterial

Mutation

contains only Cterminus (aa ~1205-1434)

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
Rabbit NHERF-1

Plasmid

#31644

Depositor

Edward Weinman

Article

Weinman et al J Clin Invest. 1995 May . 95(5):2143-9.

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
pBS-F1-GGG

Plasmid

#30703

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GGG

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F1-GTA

Plasmid

#30705

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GTA

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F1-GTC

Plasmid

#30706

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GTC

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only