We narrowed to 8,865 results for: BLI;

1. NanoBRET Assay Vector, CRAF RBD-CRD-HaloTag

   Plasmid

   #236873

   Purpose

   Express CRAF RBD-CRD-HaloTag(R) in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   CRAF (RAF1 Human)
   

   Use

   Tags

   HaloTag (R)

   Expression

   Mammalian

   Mutation

   Ras Binding Domain-Cysteine Rich Domain

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. NanoBRET Assay Vector, NanoLuc-NRAS WT

   Plasmid

   #236875

   Purpose

   Express NanoLuc(R)-NRAS WT in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   NRAS (NRAS Human)
   

   Use

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGL4 [luc2P/RBP-Jk-RE/Hygro] Vector

   Plasmid

   #236804

   Purpose

   Express RBP-Jk response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   RBP-Jk (RBPJ Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   minP

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. PiggyBac-TDP-43deltaNLS-V5-APEX

   Plasmid

   #229081

   Purpose

   Doxycycline-inducible expression of NLS-mutated TDP-43 fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone

   Depositor

   Eran Hornstein

   Insert

   TDP-43deltaNLS (TARDBP Human)
   

   Use

   Tags

   APEX, BFP, and V5

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A, K95A, K97A and R98A for mutatio…

   Promoter

   TRE3G

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pA-CBh.TP-DCP2-V5H6.MCh.Puro

   Plasmid

   #209948

   Purpose

   In mammalian cells expresses TP fused to a DCP2 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

   Depositor

   Kevin Weeks

   Inserts

   Decapping protein 2 (DCP2 Human)
   mCherry fluorescent protein fused to puromycin resistance marker
   

   Use

   Tags

   TP (MS2 coat protein) and V5 epitope with H6 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and CMV

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pA-CBh.TP-CNOT6-V5H6.MCh.Puro

   Plasmid

   #209944

   Purpose

   In mammalian cells expresses TP fused to a CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

   Depositor

   Kevin Weeks

   Inserts

   CCR4-NOT transcription complex subunit 6 (CNOT6 Human)
   mCherry fluorescent protein fused to puromycin resistance marker
   

   Use

   Tags

   TP (MS2 coat protein) and V5 epitope with H6 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and CMV

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pA-CBh.TP-CNOT6L-V5H6.MCh.Puro

   Plasmid

   #209945

   Purpose

   In mammalian cells expresses TP fused to a CNOT6L with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

   Depositor

   Kevin Weeks

   Inserts

   CNOT6L (CNOT6L Human)
   mCherry fluorescent protein fused to puromycin resistance marker
   

   Use

   Tags

   TP (MS2 coat protein) and V5 epitope with H6 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and CMV

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAVLINKv1-pCALM1-Cre-3' NEXMIF

   Plasmid

   #236669

   Purpose

   3' AAVLINK plasmid for NEXMIF expression

   Depositor

   Zhonghua Lu

   Insert

   NEXMIF (NEXMIF Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   pCALM1

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAVLINKv1-pCALM1-Cre-3' KDM5C

   Plasmid

   #236671

   Purpose

   3' AAVLINK plasmid for KDM5C expression

   Depositor

   Zhonghua Lu

   Insert

   KDM5C (KDM5C Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   pCALM1

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAVLINKv1-pCALM1-Cre-3' KDM5B

    Plasmid

    #236649

    Purpose

    3' AAVLINK plasmid for KDM5B expression

    Depositor

    Zhonghua Lu

    Insert

    KDM5B (KDM5B Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAVLINKv1-pCALM1-Cre-3' KDM5A

    Plasmid

    #236711

    Purpose

    3' AAVLINK plasmid for KDM5A expression

    Depositor

    Zhonghua Lu

    Insert

    KDM5A (KDM5A Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAVLINKv1-pCALM1-Cre-3' ITSN1

    Plasmid

    #236717

    Purpose

    3' AAVLINK plasmid for ITSN1 expression

    Depositor

    Zhonghua Lu

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAVLINKv1-pCALM1-Cre-3' KANK1

    Plasmid

    #236681

    Purpose

    3' AAVLINK plasmid for KANK1 expression

    Depositor

    Zhonghua Lu

    Insert

    KANK1 (KANK1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAVLINKv1-pCALM1-Cre-3' KDM6A

    Plasmid

    #236689

    Purpose

    3' AAVLINK plasmid for KDM6A expression

    Depositor

    Zhonghua Lu

    Insert

    KDM6A (KDM6A Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGlomyc-Grhl1 (618 aa)

    Plasmid

    #236049

    Purpose

    Mammalian expression vector with myc-tagged mouse Grhl1 coding sequence (long isoform)

    Depositor

    Renee van Amerongen

    Insert

    Grhl1 (Grhl1 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGlomyc-Grhl2

    Plasmid

    #236050

    Purpose

    Mammalian expression vector with myc-tagged mouse Grhl2 coding sequence

    Depositor

    Renee van Amerongen

    Insert

    Grhl2 (Grhl2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-IARS2-tb

    Plasmid

    #232340

    Purpose

    Expresses human IARS2 with synonymous mutations (to prevent recognition by siRNA) in mammalian cells

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 synonymous mutations in the siRNA (ACUUGCAGUCAU…

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-shortSUMO1

    Plasmid

    #228951

    Purpose

    express a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimized

    Depositor

    Marcin Suskiewicz

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGSSHHHHHHS…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-17

    Promoter

    T7-lac

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-RANGAP1-CTD

    Plasmid

    #228947

    Purpose

    express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized

    Depositor

    Marcin Suskiewicz

    Insert

    RANGAP1-CTD (RANGAP1 Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGSSHHHHHHS…

    Expression

    Bacterial

    Mutation

    contains aa 418-587 only

    Promoter

    T7-lac

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV_Mex3a_mutRING_t2a_mCherry

    Plasmid

    #223245

    Purpose

    Expression of mutant RING Mex3a in mammalian cells with t2a cleaved mCherry

    Depositor

    Stavros Lomvardas

    Insert

    Mex3a (Mex3a Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    4 point mutations in RING Domain: Cys 484 Ala; Hi…

    Promoter

    CMV

    Available since

    May 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLD-puro-Cc-IFIT5_K197R-VA

    Plasmid

    #191223

    Purpose

    Lentiviral expression of human IFIT5_K197R in mammalian cells

    Depositor

    Mohan Babu

    Insert

    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    changed Lysine 197 to Arginine (K197R)

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLD-puro-Cc-SARS-CoV-2_Spike_Alpha-VA

    Plasmid

    #191217

    Purpose

    Lentiviral expression of SARS-CoV-2 Spike_Alpha in mammalian cells

    Depositor

    Mohan Babu

    Insert

    SPIKE_SARS2_Alpha (S SARS CoV-2)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    Del 69-70 Del 144 N501Y A570D D614G P681H T716I S…

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLD-puro-Cc-SARS-CoV-2_Spike_Beta-VA

    Plasmid

    #191218

    Purpose

    Lentiviral expression of SARS-CoV-2 Spike_Beta in mammalian cells

    Depositor

    Mohan Babu

    Insert

    SPIKE_SARS2_Beta (S SARS CoV-2)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    D80A D215G (Del 241-243) K417N E484K N501Y D614G …

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLD-puro-Cc-SARS-CoV-2_Spike_Delta-VA

    Plasmid

    #191219

    Purpose

    Lentiviral expression of SARS-CoV-2 Spike_Delta in mammalian cells

    Depositor

    Mohan Babu

    Insert

    SPIKE_SARS2_Delta (S SARS CoV-2)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    T19R 156del 157del R158G L452R T478K D614G P681R …

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA

    Plasmid

    #191220

    Purpose

    Lentiviral expression of SARS-CoV-2 Spike_Lambda in mammalian cells

    Depositor

    Mohan Babu

    Insert

    SPIKE_SARS2_Lambda (S SARS CoV-2)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    G75V T76I Del 246-252 L452Q F490S D614G T859N

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLD-puro-Cc-IFIT5_K160R-VA

    Plasmid

    #191222

    Purpose

    Lentiviral expression of human IFIT5_K160R in mammalian cells

    Depositor

    Mohan Babu

    Insert

    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    changed Lysine 160 to Arginine (K160R)

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid

    #191212

    Purpose

    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay

    Depositor

    Mohan Babu

    Insert

    SARS-CoV-2 spike_WT (S SARS CoV-2)
    

    Use

    Tags

    HiBiT (high affinity small nanoluciferase fragmen…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 Flag-TUBG2

    Plasmid

    #229855

    Purpose

    TUBG2

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

    Insert

    TUBG2 (TUBG2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Removal of the internal TUBG2-EcoRI site by chang…

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HaloTag-LacI-mHP1a

    Plasmid

    #232637

    Purpose

    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alpha

    Depositor

    Karsten Rippe

    Insert

    HaloTag-LacI-mHP1a (Cbx5 Mouse, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Kif1A-EGFP-SNAP

    Plasmid

    #229851

    Purpose

    Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

    Depositor

    William Hancock

    Insert

    Kif1A-EGFP-SNAP (Kif1a Rat)
    

    Use

    Tags

    6xHis, EGFP, and SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Galt1-HT7-mGold

    Plasmid

    #230027

    Purpose

    Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Galt1-HaloTag7-mGold (B4GALT1 Human, Synthetic)
    

    Use

    Tags

    HaloTag,mGold

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid

    #222016

    Purpose

    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    PHOSPHO2 (PHOSPHO2 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    chicken β-actin promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEX-PHOSPHO2-TEV-Twin-Strep

    Plasmid

    #222017

    Purpose

    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli

    Depositor

    Chiaki Murakami

    Insert

    PHOSPHO2 (PHOSPHO2 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    deleted stop codon (TAA)

    Promoter

    lac promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AICSDP-167:CDH5-mEGFP

    Plasmid

    #217425

    Purpose

    Homology arms and mEGFP sequence for C-terminus tagging of human cadherin 5

    Depositor

    Allen Institute for Cell Science

    Insert

    CDH5 Homology Arms with mEGFP (CDH5 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP

    Expression

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pCDB179-Cut190*SS

    Plasmid

    #218706

    Purpose

    Encodes codon optimized Cut190*SSenzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    Cut190*SS
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCDB179-LCC-ICCG

    Plasmid

    #218687

    Purpose

    Encodes codon optimized LCC-ICCG enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDB179-SfCut

    Plasmid

    #218708

    Purpose

    Encodes codon optimized SfCut enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    SfCut
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-MCS-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225704

    Purpose

    Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1A

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti-MCS-3AID-HA-2A-mCherryBSD

    Plasmid

    #225700

    Purpose

    Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1A

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti-rTetR(SE)-VP64-2A-mCherryBSD

    Plasmid

    #225695

    Purpose

    Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLenti-rTetR(SE)-KRAB-2A-mTurquoise

    Plasmid

    #225690

    Purpose

    Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-ZNF10 KRAB
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET50delCT_Vipp1_dL10Ala

    Plasmid

    #223817

    Purpose

    Bacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage site

    Depositor

    Carsten Sachse

    Article

    Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

    Insert

    Vipp1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    replacement of 10 aa in Hinge 2 with Ala

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

    Plasmid

    #225442

    Purpose

    BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.

    Depositor

    Diego Orzaez

    Insert

    LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

    Plasmid

    #225441

    Purpose

    Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

    Depositor

    Diego Orzaez

    Insert

    hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSN667

    Plasmid

    #219963

    Purpose

    Expressed sfGFP fused human KIF1Bß(1-721) in insect cells

    Depositor

    Shinsuke Niwa

    Insert

    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    

    Use

    Tags

    sfGFP::2xStrepII tag

    Expression

    Insect

    Mutation

    encodes 1-721

    Promoter

    polyhedrin

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. MARS-nGFP

    Plasmid

    #205234

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    

    Use

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCDNA3-2xMARS-PLD

    Plasmid

    #205237

    Purpose

    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

    Depositor

    Jeremy Baskin

    Inserts

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    

    Use

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pSN668

    Plasmid

    #219962

    Purpose

    Expressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cells

    Depositor

    Shinsuke Niwa

    Insert

    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    

    Use

    Tags

    sfGFP::2xStrepII tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald

    Plasmid

    #214469

    Purpose

    Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. TCF4 - P381L in pENTR

    Plasmid

    #216570

    Purpose

    gateway cloning donor vector containing TCF4 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P381L

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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