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pGEL682

Plasmid

#205591

Purpose

PE2 for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL683

Plasmid

#205593

Purpose

Cas9-PE2 for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBCC078

Plasmid

#202212

Purpose

Level1 DNA-Tag with bc066

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc066

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBCC075

Plasmid

#202210

Purpose

Level1 DNA-Tag with bc063

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc063

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBCC077

Plasmid

#202211

Purpose

Level1 DNA-Tag with bc065

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc065

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK201

Plasmid

#207131

Purpose

Type 2 ptac promoter part

Depositor

Nicholas Shikuma

Article

Alker et al mBio. 2023 Aug 2:e0150223. doi: 10.1128/

Insert

ptac promoter

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T3_gRNA

Plasmid

#199389

Purpose

pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T2_gRNA

Plasmid

#197647

Purpose

pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type II sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T1_gRNA

Plasmid

#199332

Purpose

pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type I sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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Sha3Cas9

Plasmid

#192136

Purpose

Expresses Sha3Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sha3Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-133-HiBiT

Plasmid

#198313

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-133-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-289-HiBiT

Plasmid

#198311

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-289-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-190-HiBiT

Plasmid

#198312

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-190-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnfΔpvc15

Plasmid

#198284

Purpose

Produces PVCs lacking payloads by elimination of the payload loader (pvc15)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc15

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6Biso1_1219-1723_K

Plasmid

#146770

Purpose

Bacterial Expression of HsTNRC6Biso1_1219-1723

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6Biso1_1219-1723 (TNRC6B Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pUCIDT-Washington

Plasmid

#132758

Purpose

Encodes an iconic representation of a portrait of George Washington

Depositor

Mark Bathe

Article

Banal et al Nat Mater. 2021 Sep;20(9):1272-1280. doi

Insert

Washington1

Use

Unspecified

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUCIDT-Lincoln

Plasmid

#132757

Purpose

Encodes an iconic representation of a portrait of Abraham Lincoln

Depositor

Mark Bathe

Article

Banal et al Nat Mater. 2021 Sep;20(9):1272-1280. doi

Insert

Lincoln1

Use

Unspecified

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHOXB5.1.0-gDNA

Plasmid

#113783

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HOXB5

Depositor

Kevin White

Insert

HOXB5 (HOXB5 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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p236_LTJ_sgRNAFoxp3sf/J

Plasmid

#82676

Purpose

sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 sf/J

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p240_LTJ_sgRNAFoxp3K276X

Plasmid

#82675

Purpose

sgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 K276X

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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