We narrowed to 11,492 results for: ANG

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LentiGuide-Puro-CTRLg1 (CG509)

Plasmid

#139455

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-GFPg1 (BB09)

Plasmid

#139461

Purpose

Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

GFP-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP8_nostop

Plasmid

#149311

Purpose

Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LentiV_Neo_Sik3_T142Q

Plasmid

#119973

Purpose

Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Sik3 (Sik3 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

change Threonine 142 to Glutamine

Promoter

EFS promoter

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

Plasmid

#114087

Purpose

U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for FnCas12a crRNAs

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAN19-3xFLAG-ccvTIR1-NOSt

Plasmid

#108546

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Mutation

Changed F79 to G

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta7

Plasmid

#100884

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 668-781aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta6

Plasmid

#100883

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 489-781aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta5

Plasmid

#100882

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 400-666aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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CDK5 gRNA (BRDN0001145540)

Plasmid

#77542

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK5 (CDK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK4 gRNA (BRDN0001147537)

Plasmid

#76177

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK4 (CDK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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mRuby2-H2B-C-10

Plasmid

#55898

Purpose

Localization: Nucleus/Histones, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

H2B (H2BC11 Human)

Tags

mRuby2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pFIN-EF1a-CHER-WPRE

Plasmid

#44353

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

EF1a-CHER

Use

Lentiviral

Promoter

human elongation factor -1a (pos 2526-3969)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pKb-TnpB1

Plasmid

#215333

Purpose

ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

Depositor

Kutubuddin Molla

Article

Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

Insert

Rice codon optimized ISDra2 TnpB

Tags

Not applicable

Expression

Plant

Promoter

Rice ubiquitin promoter for TnpB and OsU3 promote…

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-NLC-DHFR-SpCas9

Plasmid

#124523

Purpose

Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NLC-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001162235)

Plasmid

#80261

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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PKD1 gRNA (BRDN0001148399)

Plasmid

#76842

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKD1 (PKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK12 gRNA (BRDN0001147156)

Plasmid

#75915

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK12 (CDK12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAX6 sgRNA7

Plasmid

#68466

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA GAL4 CREB 1-341 M1

Plasmid

#22973

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB (Creb1 Rat, GAL4-fusion)

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine of CREB

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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