We narrowed to 5,219 results for: Ado;
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Plasmid#60322PurposeThis plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.DepositorInsertNon active element (nearest TSS PHLDA1) (PHLDA1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald D40G
Plasmid#164211PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoterDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-1BPNLS-Cas9-1BPNLS-2AGFP
Plasmid#87109PurposeBPNLS-Cas9-BPNLS-2AGFP expression plasmidDepositorInsert1BPNLS-Cas9-1BPNLS-2A-EGFP
UseCRISPRTags3xFLAG (on Cas9)ExpressionMammalianPromoterCAGAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(D40G)
Plasmid#164204PurposeExpresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoterDepositorAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mcherry-ANXA11(R235Q)
Plasmid#164205PurposeExpresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
TagsCRY2oligo-mcherryExpressionMammalianMutationR235QPromoterCMVAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald R346C
Plasmid#164213PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoterDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald R235Q
Plasmid#164212PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoterDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter
Plasmid#172597PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.DepositorInsertmyc-PDGFRA-K627R (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627RPromoterCMVAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR D-TOPO-C3_38
Plasmid#60279PurposeThis plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.DepositorInsertACSL1 enhancer (ACSL1 Human)
UseGateway CloningAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hHP1gamma
Plasmid#181885PurposeSoluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-hHP1gamma (CBX3 Human, Mustard Weed)
TagsPHR-mCherryExpressionMammalianPromoterCMVAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR D-TOPO-C3_33
Plasmid#60275PurposeThis plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.DepositorInsertGLIS3 enhancer (GLIS3 Human)
UseGateway CloningAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)
Plasmid#156410PurposepCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12VDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
TagsmScarlet-IExpressionMammalianMutationmutant KRAS4A-G12VPromoterCMVAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-mHP1alpha
Plasmid#181883PurposeSoluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-mHP1alpha (Cbx5 Mustard Weed, Mouse)
TagsPHR-mCherryExpressionMammalianPromoterCMVAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)
Plasmid#156409PurposepCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12DDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
TagsmTagBFP2ExpressionMammalianMutationmutant KRAS4A-G12DPromoterCMVAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLuc-GID4
Plasmid#200878PurposeMammalian expression of human GID4 with N-terminal NanoLuciferase fusionDepositorAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28 His-MBP-TEV-BAP-Human BCKDK
Plasmid#232116PurposeFor bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.DepositorInsertBCKDK (BCKDK Human)
Tags6 x His, Biotin acceptor peptide (BAP), Maltose-B…ExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-mgp100-PuroR [M1G]
Plasmid#171802PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-mgp100-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)
Plasmid#156415Purposekdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseZebrafish transgenesisTagsmTagBFP2Mutationmutant KRAS4A-G12DPromoterkdrlAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 C3_13
Plasmid#60298PurposeThis plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.DepositorAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-hgp100-PuroR [M1G]
Plasmid#171801PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-hgp100-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only