We narrowed to 14,208 results for: CAR

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pET51b-NAD-cpLuc

Plasmid

#135628

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-cpLuc sensor

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFA-GFP

Plasmid

#133009

Purpose

pFA carrying a sfGFP gene

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

sfGFP

Tags

6xhis

Expression

Bacterial

Promoter

tetR

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD-Cyc1t (17-21)

Plasmid

#101700

Purpose

Cyc1t in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YJR048W (190 bp)]-CGCT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pTEF2 (01-11)

Plasmid

#101685

Purpose

pTEF2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMT-T-HSV2.3 TCR alpha chain

Plasmid

#74076

Purpose

gBT-I TCR a chain cDNA in pGEM-T

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha clone HSV2.3 (Tcra Mouse)

Expression

Bacterial

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBlueRIP.cOVA

Plasmid

#69595

Purpose

contains cytoplasmic form of ovalbumin (OVA) cDNA in pBlueRIP

Depositor

Francis R. Carbone

Article

Kurts et al J Exp Med. 1996 Sep 1;184(3):923-30.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET51b-NAD-Luc

Plasmid

#135627

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-Luc sensor

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pXY1-spc-ptb

Plasmid

#90489

Purpose

pXY1-spc derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

sceC

Expression

Bacterial

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 K410N

Plasmid

#87280

Purpose

Encodes Hsh155 K410N for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

K410N

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBlueRIP.OVA

Plasmid

#69597

Purpose

contains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-OVAhi and RIP-OVA lo mice)

Depositor

Francis R. Carbone

Article

Blanas et al Science. 1996 Dec 6;274(5293):1707-9.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Destination Vector

Plasmid

#125754

Purpose

Destination vector for assembling gRNA fragments in the second round of assembly.

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

pTDH3-GFPdropout-tTDH1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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Guide-Generating Vector

Plasmid

#125752

Purpose

Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

Cys4-GFPdropout-SpCas9scaffold

Use

Synthetic Biology

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pPI464

Plasmid

#113216

Purpose

generic knockin vector for C-terminal tagging with mScarlet

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mScarlet

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-8xHis

Plasmid

#101699

Purpose

8xHis in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

AATG-[8xHis]-AGCC

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-ADH2t (17-21)

Plasmid

#101701

Purpose

ADH2t in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YMR303C (376 bp)]-CGCT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-PGK1 (01-11)

Plasmid

#101689

Purpose

pPGK1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YCR012W (982bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pYC12

Plasmid

#63911

Purpose

Initial Overexpressing Vector

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pBC2FG

Plasmid

#31281

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Cry2 (Cry2 Mouse)

Tags

Flag and His

Expression

Insect

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pAW8ENdeI-cHAIAA7-spo5DSR

Plasmid

#49873

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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MS2-ecDHFR

Plasmid

#65859

Purpose

Expresses RNA-binding protein MS2 with eDHFR fusion tag

Depositor

Aaron Hoskins

Article

Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

Inserts

MS2
E. coli Dihydrofolate Reductase (folA )

Tags

HA and NLS

Expression

Bacterial and Yeast

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only