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pQC NLS mKorange IX

Plasmid

#37346

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

NLS mKusabira Orange

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pEB587

Plasmid

#22065

Depositor

Emmanuelle Bouveret

Article

Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

Type

Empty backbone

Tags

TAP

Expression

Bacterial

Available Since

Oct. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC-FLAG

Plasmid

#129494

Purpose

Expresses human TMEM206 tagged at the C-terminus with FLAG

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

Plasmid

#122084

Purpose

Express M.EcoGII fused to TRF1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-TERF1

Use

Retroviral

Tags

V5 tag on Nter of TERF1

Expression

Bacterial and Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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StrKDEL-IRES_mScarleti_Sec23A

Plasmid

#117273

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23A

Depositor

David Stephens

Article

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Inserts

SEC23A (SEC23A Human)
Streptavidin-KDEL

Tags

mScarlet-i

Expression

Mammalian

Mutation

Human Sec23A; Sequencing showed a mutation in Sec…

Promoter

CMV and IRES

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pIRES proHB-EGF NC

Plasmid

#11604

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Mutation

Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
BK beta2 EGFP

Plasmid

#113558

Purpose

human BK beta2 subunit in C-term translational frame with EGFP (EGFP-N1)

Depositor

Robert Brenner

Insert

Kcnmb2 (KCNMB2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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V37 pDNR MCS SA - Donor

Plasmid

#11690

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

July 19, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-Hygro_hCOL4A3

Plasmid

#198098

Purpose

lentiviral expression of hCOL4A3 in mammalian cells

Depositor

Jeffrey Miner

Insert

COL4A3 (COL4A3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB-LpxE

Plasmid

#128389

Purpose

Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Inserts

PglB oligosaccharylktransferase
LpxE phosphatase

Tags

Flag tag

Expression

Bacterial

Promoter

Ptac and pBAD

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pQXCINeo-LOX

Plasmid

#134761

Purpose

a retroviral vector to express lysyl oxidase (derived from PCR4-TOPO-LOX)

Depositor

Philippe Clezardin, Martine Croset

Insert

LOX (LOX Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSplit-NO-Dib1

Plasmid

#58786

Purpose

Express LucN-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDIb1

Tags

Flag and Luciferase (1-398aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
SFFV-DTAG-CTERM-GFP

Plasmid

#185765

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

SFFV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ4

Plasmid

#167133

Purpose

A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Expression

Bacterial

Promoter

Kanamycin promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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SNAP-Dcp2

Plasmid

#153975

Purpose

SNAP-tag polypeptide fused to mRNA decapping enzyme 2

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA Decapping enzyme 2 (DCP2 Human)

Tags

SNAP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
SFFV-IKZF3-CTERM-GFP

Plasmid

#185769

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-shN

Plasmid

#73665

Purpose

pSIREN-RetroQ vector containing control sequence N

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA Neg Control

Use

Retroviral

Available Since

March 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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