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Showing: 2901 - 2920 of 9949 results

  1. pVH016

    Plasmid
    #80400
    Purpose
    No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    C-terminal 3xFLAG tag and C-terminal LZx domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    80400-plasmid-map-sequence-id-155905

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  3. pLsCas13aGG

    Plasmid
    #131012
    Purpose
    Backbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of LsCas13a
    GFP expression cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131012-plasmid-map-sequence-id-258107

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  6. attB-P7-attP-YFP

    Plasmid
    #195571
    Purpose
    A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.
    Depositor
    Richard Murray
    Article
    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52
    Insert
    Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    P7
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    195571-plasmid-map-sequence-id-383991

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  9. pAsCpf1GG

    Plasmid
    #131011
    Purpose
    Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of AsCas12a
    GFP expression cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131011-plasmid-map-sequence-id-258106

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  10. pONSY

    Plasmid
    #111873
    Purpose
    "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Type
    Empty backbone
    Use
    Capsaspora owczarzaki
    Tags
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111873-plasmid-map-sequence-id-217304

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  17. pVH028

    Plasmid
    #80393
    Purpose
    Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    Taz chimeric histine kinase
    CusS (cusS )
    NahR (nahR )
    Use
    Synthetic Biology
    Tags
    Fused by GS linkers to 4 SH3 domains
    Expression
    Bacterial
    Mutation
    Glycine 448 changed to Alanine, makes it primaril…
    Promoter
    Available since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    80393-plasmid-map-sequence-id-166533

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Showing: 2901 - 2920 of 9949 results