We narrowed to 3,095 results for: sam

1. 12x PRE TK luc

   Plasmid

   #206163

   Purpose

   Luciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   12X PRE TK luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   min TK

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL3927 (pDonor(plasmid)_Pse)

   Plasmid

   #200905

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (pTarget NGS)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28C-mCherry-DDX4LCD

   Plasmid

   #204408

   Purpose

   E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   DDX4(1-236) (DDX4 Human)
   

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28C-mCherry-53BP1LCD

   Plasmid

   #204407

   Purpose

   E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   53BP1(1208-1977) (TP53BP1 Human)
   

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

   Plasmid

   #130640

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6
   

   Use

   CRISPR; Transposon

   Tags

   His10-MBP-TEVsite on TniQ

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL0828 (pQCascade_crRNA-4)

   Plasmid

   #130637

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQFmcs-2H12.D11-scarREF

   Plasmid

   #221184

   Purpose

   cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2
   mScarlet-I
   

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   None (same as cdGreen2) and PQ5 (cumate-inducible)

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL0762 (pQCascade_crRNA-nt)

   Plasmid

   #130636

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFE-Rrub-FII-prk

   Plasmid

   #162695

   Purpose

   Expresses R. rubrum Form II rubisco and S. elongatus Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Inserts

   Rubrum Form II rubisco
   S. elongatus PRK
   

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter and under same promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL0812 (pCRISPR(BbsI)_Vch)

    Plasmid

    #200898

    Purpose

    Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCRISPR (entry)
    

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHM123DEnv

    Plasmid

    #137668

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Expression

    Mammalian

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCS.71/2272

    Plasmid

    #22732

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox71-MCS-Lox2272
    

    Use

    Cre/Lox

    Mutation

    This is a basal exchange vector for RMCE containi…

    Available Since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

    Plasmid

    #130639

    Purpose

    Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on Cas8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLINE1-scFvFc

    Plasmid

    #206020

    Purpose

    An intact type LINE-1 vector plasmid possessing the scFv-Fc expression unit

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. cfmTurquoise-N

    Plasmid

    #139284

    Purpose

    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

    Depositor

    Ikuo Wada

    Article

    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

    Insert

    cfmTurquoise
    

    Tags

    Multi-cloning sites

    Expression

    Mammalian

    Mutation

    Cys48Ser and Cys70/Met

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL0373 (His-MBP-TniQ)

    Plasmid

    #130638

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIG-Flag-RIPK1-D324A

    Plasmid

    #102678

    Purpose

    retroviral expression plasmid for RIPK1-D324A

    Depositor

    Hasam Habelhah

    Article

    Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and IRES-GFP

    Expression

    Mammalian

    Mutation

    CHANGE Glutamate 324 to Alanine

    Promoter

    unknown

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL0721 (pDonor_L1-105)

    Plasmid

    #130645

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (short L)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL0376 (His-MBP-StrepII-TniQ)

    Plasmid

    #130641

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite-StrepII on TniQ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL0715 (pDonor_R1-57)

    Plasmid

    #130644

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (short R)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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