We narrowed to 52,854 results for: plasmid

1. CD44I-c001

   Plasmid

   #175448

   Purpose

   Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pH3F

   Plasmid

   #85773

   Purpose

   N-ICE plasmid pH3F

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-HphMX-loxP-3FLAG
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124287

   Purpose

   sgRNA against human Col7A1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Collagen type VII alpha 1 chain (COL7A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pGBW-m4046286

   Plasmid

   #149147

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pISlin41w(s) (lin-41 microRNA wt reporter)

   Plasmid

   #11906

   Depositor

   David Bartel

   Article

   Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

   Insert

   lin-41 miRNA target sequence (lin-41 Nematode)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJ291_WT_low

   Plasmid

   #128855

   Purpose

   Low copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p183_LTJ_sgRNACD90.2_A

   Plasmid

   #82671

   Purpose

   sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting CD90.2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMLX-donor

   Plasmid

   #112370

   Purpose

   CRISPR donor plasmid to tag human transcription factor MLX with GFP

   Depositor

   Kevin White

   Insert

   MLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:17:42570798:G>A, hg38:17:42570950:T>G,…

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4164344

   Plasmid

   #153554

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   accatg Kozak-START linker

   Expression

   Mammalian

   Mutation

   Gordon et al recoded

   Promoter

   Available since

   June 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. JARID1BA

    Plasmid

    #74648

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JARID1BA (KDM5B Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Expression

    Mutation

    Leu101-(Gly)4-Ala374 deletion mutant

    Promoter

    PH

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pJ211_rha_WT_low

    Plasmid

    #128851

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJ211_rha_WT_med

    Plasmid

    #128852

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pK3F-PYK1

    Plasmid

    #85771

    Purpose

    N-ICE plasmid pK3F-PYK1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-PYK1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124286

    Purpose

    sgRNA against human Col7A1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Collagen type VII alpha 1 chain (COL7A1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ACE2-Mutant-R652A

    Plasmid

    #240605

    Purpose

    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2; R was substituted by A at Residue 652 of ACE2 in the collectrin-like site

    Depositor

    Zaid Abassi

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Arginine (R) at position 652 on ACE2 is replaced …

    Promoter

    CMV

    Available since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SAGA046

    Plasmid

    #240795

    Purpose

    Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA pBR322ROP His medium : T7- His tags medium- gfp -pBR322ROP tetApBR322ROP - CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SAGA045

    Plasmid

    #240794

    Purpose

    Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA pBR322ROP His low : T7- His tags low - gfp -pBR322ROP tetApBR322ROP - CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SAGA043

    Plasmid

    #240792

    Purpose

    Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA p15a His medium : T7- His tags medium- gfp -p15a tetAp15a - CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SAGA042

    Plasmid

    #240791

    Purpose

    Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA p15a His low : T7- His tags low - gfp -p15a tetAp15a - CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SAGA040

    Plasmid

    #240789

    Purpose

    Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 His medium : T7- His tags medium- gfp -RK2 tetARK2 - CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SAGA039

    Plasmid

    #240788

    Purpose

    Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 His low : T7- His tags low - gfp -RK2 tetARK2 - CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SAGA032

    Plasmid

    #240781

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 pT7cons BCD_high - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SAGA031

    Plasmid

    #240780

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 pT7cons BCD_medium - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SAGA030

    Plasmid

    #240779

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 pT7cons BCD_low - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SAGA023

    Plasmid

    #240772

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 Ptrc BCD_high - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SAGA022

    Plasmid

    #240771

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 Ptrc BCD_medium - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SAGA021

    Plasmid

    #240770

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 Ptrc BCD_low - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SAGA019

    Plasmid

    #240768

    Purpose

    Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA pBR322-ROP PrhaBAD BCD_medium - gfp -tetApBR322-ROP- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SAGA018

    Plasmid

    #240767

    Purpose

    Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA pBR322-ROP PrhaBAD BCD_low - gfp - tetApBR322-ROP- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SAGA015

    Plasmid

    #240764

    Purpose

    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA p15A PrhaBAD BCD_low - gfp - tetAp15A- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SAGA014

    Plasmid

    #240763

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 PrhaBAD BCD_high - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SAGA013

    Plasmid

    #240762

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 PrhaBAD BCD_medium - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SAGA012

    Plasmid

    #240761

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 PrhaBAD BCD_low - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SAGA009

    Plasmid

    #240758

    Purpose

    Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA pBR322-ROP J23100 BCD_high - gfp - tetApBR322-ROP- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SAGA008

    Plasmid

    #240757

    Purpose

    Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA pBR322-ROP J23100 BCD_medium - gfp -tetApBR322-ROP- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SAGA007

    Plasmid

    #240756

    Purpose

    Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA pBR322-ROP J23100 BCD_low - gfp - tetApBR322-ROP- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SAGA006

    Plasmid

    #240755

    Purpose

    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA p15A J23100 BCD_high - gfp - tetAp15A- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SAGA005

    Plasmid

    #240754

    Purpose

    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA p15A J23100 BCD_medium - gfp - tetAp15A- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SAGA004

    Plasmid

    #240753

    Purpose

    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA p15A J23100 BCD_low - gfp - tetAp15A- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SAGA003

    Plasmid

    #240752

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 J23100 BCD_high - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. SAGA002

    Plasmid

    #240751

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 J23100 BCD_medium - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. SAGA001

    Plasmid

    #240750

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 J23100 BCD_low - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. ACE2-Mutant-DeltaC4

    Plasmid

    #240609

    Purpose

    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2 likely at Cluster 4 of ACE2 in the collectrin-like site. The whole C4 sequence was deleted in this mutant

    Depositor

    Zaid Abassi

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    deletion of the full Cluster 4 sequence on ACE2 (…

    Promoter

    CMV

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. ACE2-HA-Linker-FLAG

    Plasmid

    #240602

    Purpose

    Encodes doubly tagged ACE2 protein. HA on the N-terminal added after the signal peptide (a.a 1-17) and FLAG tag at the end of the insert. A linker sequence was added after the HA tag.

    Depositor

    Zaid Abassi

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    FLAG tag and HA tag (after the signal peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. EGFR(L858R,T790M)-NanoLuc Fusion Vector

    Plasmid

    #237199

    Purpose

    Express EGFR(L858R,T790M)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    L858R/T790M double mutations

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. NanoBRET Assay Vector, NanoLuc-MLL1

    Plasmid

    #237135

    Purpose

    Express NanoLuc(R)-MLL1 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MLL1 (KMT2A Human)
    

    Use

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. CRAF(RBD-CRD)-SmBiT BiBiT-Ready Vector

    Plasmid

    #237077

    Purpose

    Express CRAF(RBD-CRD)-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    Ras Binding Domain-Cysteine Rich Domain

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. NanoBRET Assay Vector, BRAF RBD-HaloTag

    Plasmid

    #236879

    Purpose

    Express BRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    Ras Binding Domain

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. NanoBRET Assay Vector, CRAF RBD-CRD-HaloTag

    Plasmid

    #236873

    Purpose

    Express CRAF RBD-CRD-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    Ras Binding Domain-Cysteine Rich Domain

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. pLD-puro-Cc-IFIT5_K197R-VA

    Plasmid

    #191223

    Purpose

    Lentiviral expression of human IFIT5_K197R in mammalian cells

    Depositor

    Mohan Babu

    Insert

    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    changed Lysine 197 to Arginine (K197R)

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge