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pMH-SFB-BRCA1

Plasmid

#99394

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1

Depositor

Junjie Chen, Michael Huen

Article

Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

BRCA1 (BRCA1 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD

Plasmid

#81053

Purpose

bacterial expression of the catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-CRY2-VHH(GFP)

Plasmid

#58370

Purpose

For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Tags

SNAP and vhhGFP4

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-T7-Keap1

Plasmid

#21552

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

kelch-like ECH-associated protein 1 (KEAP1 Human)

Tags

T7

Expression

Mammalian

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLNCX1 HA Akt1

Plasmid

#15990

Depositor

Morris Birnbaum

Article

Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-mNeongreen

Plasmid

#182387

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC3-mSufu

Plasmid

#65431

Purpose

Expresses mouse Sufu protein in mammalian cells

Depositor

Aimin Liu

Article

Zeng et al PLoS One. 2010 Dec 29;5(12):e15900. doi:

Insert

Suppressor of Fused (Sufu Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pXPR_123

Plasmid

#96919

Purpose

for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

p300

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEZY19

Plasmid

#18668

Purpose

pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Gateway Cloning

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR223-NEK9

Plasmid

#23459

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK9 (NEK9 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DYRK3

Plasmid

#23635

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DYRK3 (DYRK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pET3d::strep-AID

Plasmid

#15924

Depositor

Nina Papavasiliou

Article

Dickerson et al J Exp Med. 2003 May 19. 197(10):1291-6.

Insert

AID (AICDA Human)

Tags

strep

Expression

Bacterial

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pEBG p130cas

Plasmid

#15001

Depositor

Raymond Birge

Article

Zvara et al Oncogene. 2001 Feb 22. 20(8):951-61.

Insert

p130cas (Bcar1 Rat)

Tags

GST

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7b1(glu)

Plasmid

#14673

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1(Glu) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Ser-269Glu Thr-273Glu

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pJH200

Plasmid

#13327

Depositor

Martin Gellert

Article

Lieber et al Genes Dev. 1987 Oct . 1(8):751-61.

Insert

recombination substrate

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pLL3.7m-psMEK1tight

Plasmid

#89362

Purpose

A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1tight (MAP2K1 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2 GFP-Rtn4a

Plasmid

#86684

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pNPK-NLS-Vika

Plasmid

#79969

Purpose

expresses human codon-optimized Vika-recombinase in mammalian cells

Depositor

Frank Buchholz

Article

Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

Insert

human codon-optimized Vika sequence, NLS tagged

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM24

Plasmid

#65396

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM24 (TRIM24 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pFA6a-link-GFPEnvy-SpHis5

Plasmid

#60782

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPEnvy using SpHis5 selection

Depositor

Linda Huang

Article

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPEnvy

Use

Yeast integrating

Mutation

S30R Y39N T65C S72A F99S N105T Y145F M153T V163A …

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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