We narrowed to 4,495 results for: Ado

1. pLenti PA-mCit

   Plasmid

   #113451

   Purpose

   Lentiviral ProteinA-mCitrine expression vector

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-mCitrine
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBbC

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-HyPer-DAAO-NES

   Plasmid

   #119164

   Purpose

   Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.

   Depositor

   Thomas Michel

   Article

   Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

   Insert

   HyPer-DAAO-NES
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

   Plasmid

   #194279

   Purpose

   Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

   Inserts

   humanized VP64 dSaCas9 VP64 T2A Thy1.1
   gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and hUbC

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

   Plasmid

   #176627

   Purpose

   TF B self-activation cassette in MultiFate-2.2 and MultiFate-3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-ihSyn1-DIO-tTA

   Plasmid

   #99121

   Purpose

   An AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTA

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   DIO-tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   ihSyn1

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.4_CEACAM6-His

   Plasmid

   #149337

   Purpose

   for eukaryotic expression and purification of CEACAM6-His6

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Use

   Tags

   IL-2 signal peptide and polyhistidine

   Expression

   Mammalian

   Mutation

   point mutation (GGA to GCA) to replace 'G-g…

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a_NRL_P2A_Hygro_Barcode

   Plasmid

   #120469

   Purpose

   Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   NRL (NRL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEE075

   Plasmid

   #176832

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   714
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-ChRmine-eYFP-WPRE

   Plasmid

   #130988

   Purpose

   AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Expression

    Mutation

    codon optimized for human

    Promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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