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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Article

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Use

Tags

GST Tag

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pYL322d1/N-eGFP

Plasmid

#183151

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/N-Vc

Plasmid

#183157

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S

Plasmid

#211837

Purpose

Plant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Mutation

Promoter

UBIQUITIN10

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL685

Plasmid

#205592

Purpose

NEPE for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

ZmUbi

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIK

Plasmid

#126554

Purpose

Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaE (pESC124)

Plasmid

#50390

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

DPCK

Use

Tags

6XHIS tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pMOD_C0000

Plasmid

#91081

Purpose

Module C, Promoter: none, Gene: none (BaeI site for GT donor cloning), Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB701NL3F10H

Plasmid

#141288

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B0000

Plasmid

#91058

Purpose

Module B, Promoter: none, Gene: none, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-t35s

Plasmid

#118385

Purpose

Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19/Vc-C

Plasmid

#183158

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFH66

Plasmid

#131765

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A0000

Plasmid

#90997

Purpose

Module A, Promoter: none, Gene: none, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pYL322d1/eGFP-C

Plasmid

#183152

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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CDS_mALS

Plasmid

#136081

Purpose

Confers plant resistance to chlorsulfuron

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_mALS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDGB3_alpha1-AtUBQ10:GFP-T35S

Plasmid

#211836

Purpose

Plant transient expression cassette for GFP (control) under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

GFP control

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Mutation

Promoter

UBIQUITIN10

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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p221z-dCAS9p-t35s

Plasmid

#118387

Purpose

Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

dCAS9p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A3003

Plasmid

#91043

Purpose

Module A, Promoter: CmYLCV, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

CmYLCV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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