We narrowed to 11,559 results for: NSI

1. pHH0103_VAV1-2/2

   Plasmid

   #91275

   Purpose

   Protein expression and purification of human SH3 domain construct VAV1-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   VAV1-2/2 (VAV1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBad-CA TAG186

   Plasmid

   #105837

   Purpose

   Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 186

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBad-CAts TAG97

   Plasmid

   #105668

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 97

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-R1ACR_877-EYFP

   Plasmid

   #103883

   Purpose

   Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin R1ACR_877
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-R1ACR_447-EYFP

   Plasmid

   #103960

   Purpose

   Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin R1ACR_447
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-R2ACR_853-EYFP

   Plasmid

   #103963

   Purpose

   Anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin R2ACR_853
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-C1ACR_023-EYFP

   Plasmid

   #103966

   Purpose

   Anion channelrhodopsin from unclassified cryptophyte (CCMP2293) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin C1ACR_023
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-R1ACR_799-EYFP

   Plasmid

   #103962

   Purpose

   Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin R1ACR_799
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-C1ACR_887-EYFP

   Plasmid

   #103965

   Purpose

   Anion channelrhodopsin from unclassified cryptophyte (CCMP2293) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin C1ACR_887
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-G1ACR_203-EYFP

    Plasmid

    #103143

    Purpose

    Anion channelrhodopsin from Geminigera sp. (Caron Lab Isolate) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin G1ACR_203
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-GcACR_145-EYFP

    Plasmid

    #103138

    Purpose

    Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin GcACR_145
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-R1ACR_367-EYFP

    Plasmid

    #103773

    Purpose

    Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R1ACR_367
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-GcACR_439-EYFP

    Plasmid

    #103142

    Purpose

    Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin GcACR_439
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p415TEF-NYFP

    Plasmid

    #99555

    Purpose

    Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

    Depositor

    Liming Li

    Article

    Hines et al PLoS Genet. 2011 Feb;7(2):e1001309. doi:

    Insert

    Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)

    Plasmid

    #91874

    Purpose

    Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Insert

    Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLhE_AW-pHG165

    Plasmid

    #90062

    Purpose

    Constitutive expression of a chimeric LacI:CelR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhE_AW
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I48V/Q55A/Q60R

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLhS-pHG165a

    Plasmid

    #90040

    Purpose

    Constitutive expression of a chimeric LacI:GalS transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LLhF-pHG165a

    Plasmid

    #90041

    Purpose

    Constitutive expression of a chimeric LacI:FruR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LLhT-pHG165a

    Plasmid

    #90042

    Purpose

    Constitutive expression of a chimeric LacI:TreR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLhR-pHG165a

    Plasmid

    #90043

    Purpose

    Constitutive expression of a chimeric LacI:RbsR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LLhA-pHG165a

    Plasmid

    #90044

    Purpose

    Constitutive expression of a chimeric LacI:AscG transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LLhE-pHG165

    Plasmid

    #90045

    Purpose

    Constitutive expression of a chimeric LacI:CelR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LLhC-pHG165a

    Plasmid

    #90046

    Purpose

    Constitutive expression of a chimeric LacI:CytR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKDsgRNA-srlA

    Plasmid

    #89965

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    srlA gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKDsgRNA-rbsK

    Plasmid

    #89963

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rbsK gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKDsgRNA-yhhT

    Plasmid

    #89958

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    yhhT gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKDsgRNA-torZ

    Plasmid

    #89956

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    torZ gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKDsgRNA-arsB

    Plasmid

    #89952

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    arsB gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTCW018

    Plasmid

    #83531

    Purpose

    Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Insert

    pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pARO9/pFUS1J2

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR-IAA27_no stop

    Plasmid

    #83860

    Purpose

    Gateway donor vector for IAA27 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA27 (PAP2 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR-IAA8_no stop

    Plasmid

    #83874

    Purpose

    Gateway donor vector for IAA8 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    indole-3-acetic acid inducible 8 (IAA8 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR-IAA5_no stop

    Plasmid

    #83868

    Purpose

    Gateway donor vector for IAA5 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA5 (IAA5 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR-IAA3_no stop

    Plasmid

    #83863

    Purpose

    Gateway donor vector for IAA3 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA3 (SHY2 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDONR-IAA10_no stop

    Plasmid

    #83848

    Purpose

    Gateway donor vector for IAA10 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR-IAA4_no stop

    Plasmid

    #83866

    Purpose

    Gateway donor vector for IAA4 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA4 (ATAUX2-11 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR-IAA28_no stop

    Plasmid

    #83861

    Purpose

    Gateway donor vector for IAA28 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    NP_568478.1 (IAA28 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR-IAA15_no stop

    Plasmid

    #83853

    Purpose

    Gateway donor vector for IAA15 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    indole-3-acetic acid inducible 15 (IAA15 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TU#1805_CRISPR_unc-73_exon2

    Plasmid

    #82359

    Purpose

    to create unc-73B null allele

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDGB1alpha1_SF (GB0106)

    Plasmid

    #68232

    Purpose

    Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTAct2 (GB0210)

    Plasmid

    #68191

    Purpose

    Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtAct2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge