We narrowed to 5,891 results for: dna plasmids

1. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

   Plasmid

   #167296

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAX (BAX Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiV2-mU6-evopreQ1

   Plasmid

   #210190

   Purpose

   pegRNA/ngRNA library cloning

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK2-let-7 WT

   Plasmid

   #78260

   Purpose

   psiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   fully complimentary let-7 target site
   

   Use

   Luciferase; Microrna activity

   Expression

   Mammalian

   Available Since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-BE4-SsAPOBEC3B

   Plasmid

   #138343

   Purpose

   Mammalian expression plasmid for BE4-SsAPOBEC3B

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-SsAPOBEC3B
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-BE4-PpAPOBEC1

   Plasmid

   #138349

   Purpose

   Mammalian expression plasmid for BE4-PpABOBEC1

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-PpAPOBEC1
   

   Tags

   BP-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-BE4-AmAPOBEC1

   Plasmid

   #138342

   Purpose

   Mammalian expression plasmid for BE4-AmABOBEC1

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-AmAPOBEC1
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-BE4-RrA3F

   Plasmid

   #138340

   Purpose

   Mammalian expression plasmid for BE4-RrA3F

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-RrA3F
   

   Tags

   BP-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-His-VCre

   Plasmid

   #123131

   Purpose

   Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   VCre recombinase
   

   Use

   Cre/Lox

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGK61

   Plasmid

   #228121

   Purpose

   Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem

    Plasmid

    #229792

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

    Plasmid

    #39454

    Depositor

    Hongjun Song

    Article

    Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

    Insert

    HA-hTet1CD (TET1 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1418–2136

    Promoter

    EF1a

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

12. eGFPbait-E2A-KalTA4-pA donor vector

    Plasmid

    #61069

    Purpose

    for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

    Depositor

    Filippo Del Bene

    Article

    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

    Inserts

    eGFPbait
    KalTA4
    

    Use

    Zebrafish expression

    Tags

    E2A

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-shRNA-Tet2

    Plasmid

    #85743

    Purpose

    shRNA against mouse Tet2

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA Tet2
    

    Use

    AAV

    Tags

    EYFP

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. psiCHECK2-miR-34 WT

    Plasmid

    #78258

    Purpose

    psiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    fully complimentary miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Expression

    Mammalian

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-shRNA-Tet1

    Plasmid

    #85742

    Purpose

    shRNA against mouse Tet1

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA Tet1
    

    Use

    AAV

    Tags

    EYFP

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHJ4: LexA-VHH-Caffeine

    Plasmid

    #108242

    Purpose

    LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    LexA DNA binding domain fusing with VHH-Caffeine antibody
    

    Use

    Synthetic Biology

    Promoter

    pLacO1

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGK112

    Plasmid

    #228127

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-BE4-SsAPOBEC3B R54Q

    Plasmid

    #138344

    Purpose

    Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-SsAPOBEC3B R54Q
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLdCH

    Plasmid

    #84291

    Purpose

    Express gRNA and Cas9 in Leishmania with Hygromycin resistance

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

    Insert

    gRNA and Cas9
    

    Use

    CRISPR; Leishmania

    Promoter

    L. donovani ribosome RNA promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLP-mScarlet-I

    Plasmid

    #209993

    Purpose

    Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    mScarlet-I
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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