We narrowed to 7,351 results for: ski

1. pRS415-TEF1pr-NFAPorig

   Plasmid

   #221086

   Purpose

   FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS415-TEF1pr-CFAPorig

   Plasmid

   #221087

   Purpose

   FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS19

   Plasmid

   #229475

   Purpose

   Expresses RssB with E135A, E136A, & E137A mutation

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (E135A, E136A, E137A)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E135A, E136A, E137A

   Promoter

   T7

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS21

   Plasmid

   #229476

   Purpose

   Expresses RssB with E135A, E136A, & E137A mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (E135A, E136A, E137A)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   E135A, E136A, E137A

   Promoter

   T7

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVF18

   Plasmid

   #229456

   Purpose

   Expresses IraD (aa17-130) with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   IraD (aa 17-130 only)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 17-130 only

   Promoter

   T7

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM201

   Plasmid

   #227660

   Purpose

   pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

   Depositor

   Andreas Martin

   Article

   Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

   Insert

   rpn5 (RPN5 Budding Yeast)
   

   Use

   Tags

   3x-FLAG-HRV3C-

   Expression

   Yeast

   Mutation

   (amino acids 121-131) VTENKIF mutated to AAAAAAA

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TCT-Universal plasmid

   Plasmid

   #221852

   Purpose

   A universal plasmid for expressing any protein with TetraCysteine motif

   Depositor

   Artur Krężel

   Article

   Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

   Insert

   Tetra Cysteine tag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRAR119

   Plasmid

   #185051

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L34 coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L34
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. RT-5

   Plasmid

   #220442

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (puf-3 Nematode)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RT-4

    Plasmid

    #220443

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (PUF3 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-MBP-TEVpCS-ADGRF2 CTF-FLAG

    Plasmid

    #217695

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF2 (ADGRF2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-429

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-MBP-TEVpCS-ADGRE4 CTF-FLAG

    Plasmid

    #217692

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE4 (ADGRE4P Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-173

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-MBP-TEVpCS-ADGRA1-FLAG

    Plasmid

    #217678

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA1 (ADGRA1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 3'-BirA*-FLAG minimal CMV pCDNA5

    Plasmid

    #212047

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 5'-BirA*-FLAG minimal CMV pCDNA5

    Plasmid

    #212046

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 5'-3xFLAG GFP minimal CMV pCDNA5

    Plasmid

    #212086

    Purpose

    LR vector for integration of GFP into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221-kcnk2a

    Plasmid

    #208983

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk2a (kcnk2a Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL0_DE-Per_sig_pep

    Plasmid

    #202152

    Purpose

    Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Peroxisome signal peptide
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCW-LIC-N8C_Fab5a

    Plasmid

    #203693

    Purpose

    Expresses N8C_Fab5a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab5a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCW-LIC-N8C_Fab2a

    Plasmid

    #203688

    Purpose

    Expresses N8C_Fab2a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab2a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge