165,343 results

1. pMCB320

   Plasmid

   #89359

   Purpose

   3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   puromycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

   Plasmid

   #44362

   Purpose

   Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV; Adeno associated viral vector

   Tags

   mCherry

   Promoter

   human Synapsin 1

   Available Since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-PGK-Neo-PIP-FUCCI

   Plasmid

   #118616

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. murine TCR OTI-2A.pMIG II

   Plasmid

   #52111

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   T2A (from Thosea asigna virus)

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-Ii_VSVG-SBP-mCherry

   Plasmid

   #65301

   Purpose

   synchronize trafficking of VSVG from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   VSV-G
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1A-CD19.28H.28TM.28.3z

   Plasmid

   #200678

   Purpose

   Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EZ-Tet-pLKO-Hygro

   Plasmid

   #85972

   Purpose

   Tet/Dox inducible shRNA lentivirus with hygromycin selection

   Depositor

   Cindy Miranti

   Article

   Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Promoter

   H1 / TetO

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-puro

   Plasmid

   #39481

   Purpose

   Tetracycline inducible 3rd generation lentiviral vector

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5 and 6XHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-ChrimsonR-tdT

    Plasmid

    #59169

    Purpose

    Expression vector for ChrimsonR-tdTomato

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    CAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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