We narrowed to 10,838 results for: ENA

1. pEGFP-parkin WT

   Plasmid

   #45875

   Depositor

   Edward Fon

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi_RP_3XNLS-MicroID2-FLAG

   Plasmid

   #188523

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   3XNLS-MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_816

   Plasmid

   #201157

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D908A

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

   Plasmid

   #153005

   Purpose

   Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (C) (G79)
   

   Use

   Lentiviral

   Tags

   FRB, HA, and HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBHM2329

   Plasmid

   #171686

   Purpose

   pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   C. neoformans U6

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

   Plasmid

   #153004

   Purpose

   Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (N) (G78)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-TDsmURFP-IRES-HO-1

   Plasmid

   #80346

   Purpose

   Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   HO-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and IRES

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF-XMAS-1xStop

   Plasmid

   #164411

   Purpose

   Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-HSPA8

   Plasmid

   #121161

   Purpose

   Mammalian expression of GFP tagged HSPA8

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   HSPA8 (HSPA8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

    Plasmid

    #185686

    Purpose

    Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-HaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-CMV-CD19 puro

    Plasmid

    #196634

    Purpose

    Express CD19

    Depositor

    Nicolas Manel

    Article

    Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

    Insert

    CD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPR-Cas3

    Plasmid

    #209427

    Purpose

    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; crRNA under control of PermE*

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPUR-mU6-sgRNA-2XPP7

    Plasmid

    #194504

    Purpose

    Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-F in pAR-9

    Plasmid

    #166921

    Purpose

    AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F

    Depositor

    Casey Maguire

    Article

    Hanlon et al Mol Ther Methods Clin Dev. 2019 Oct 23;1

    Insert

    REP/CAP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SP1-SB100X

    Plasmid

    #154887

    Purpose

    Sleeping Beauty transposase fused to SP1

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    SP1-Sleeping Beauty
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBA407

    Plasmid

    #85970

    Purpose

    UPRE-mCh-Ubc-Neo

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    mCherry
    Neo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-HP1beta

    Plasmid

    #17651

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 beta (CBX1 Human)
    

    Use

    Tags

    GFP and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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