We narrowed to 24,746 results for: fer

1. Luc-pSUPER-Retro-GFP/Neo

   Plasmid

   #22133

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   synthethic oligonucleotide
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   synthethic oligonucleotide encoding shRNA targeti…

   Promoter

   Available since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.R_TRE_minCMV

   Plasmid

   #211518

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SV40 and minCMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPC-Flag-PML-IV

   Plasmid

   #62804

   Purpose

   expresses the isoform IV of PML (promyelocytic leukemia protein)

   Depositor

   Gerardo Ferbeyre

   Article

   Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

   Insert

   PML-IV (PML Human)
   

   Use

   Retroviral

   Tags

   3X-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

4. RabV CVS-N2c(deltaG)-tdTomato

   Plasmid

   #73462

   Purpose

   Expresses tdTomato for monosynaptic circuit mapping

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   tdTomato
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-mArg1 base promoter -31/-2365

   Plasmid

   #34570

   Depositor

   Peter Murray

   Article

   Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

   Insert

   Arg1 base promoter -31/-2365 (Arg1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pEHlyA5

   Plasmid

   #168298

   Purpose

   Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

   Depositor

   Luis Ángel Fernández

   Article

   Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

   Insert

   N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
   

   Use

   Tags

   E-tag, HA-tag, His-tag, Prescission site, and VHH…

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1-Rheb

   Plasmid

   #133767

   Purpose

   Expresses GFP-tagged Rheb in mammalian cells

   Depositor

   Shawn Ferguson

   Article

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Insert

   Rheb (RHEB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. c-myc promoter (TBE 1/2 - wt)

   Plasmid

   #16595

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (TBE 1/2 wt) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pSEVA221pmCDA1-T7RNAP

   Plasmid

   #167978

   Purpose

   Expresses fusion protein pmCDA1-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   pmCDA1-T7RNAP (T7p07 Synthetic)
   

   Use

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-Ptet

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1-H-Luc_nucl

    Plasmid

    #113931

    Purpose

    Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc_nucl
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1-H-Luc_cyto

    Plasmid

    #113929

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL6

    Plasmid

    #48746

    Purpose

    Expression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    mPer2 promoter/enhancer region (-1128 to +2129)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pdCas9-J23111

    Plasmid

    #113149

    Purpose

    Plasmid constitutively expressing dCas9 protein

    Depositor

    Chong Zhang

    Article

    Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A & H840A

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

    Plasmid

    #159583

    Purpose

    Luciferase reporter for ribosome pausing

    Depositor

    Shintaro Iwasaki

    Article

    Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

    Inserts

    XBP1u pause site
    EIF2S3 5UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Spacer Switch Plasmid

    Plasmid

    #215346

    Purpose

    Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    OpaD spacer; Chloramphenicol Resistance Cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

    Plasmid

    #105234

    Purpose

    Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Bacterial

    Mutation

    D10A, H599A

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-REPORT (PGK) NanoLuc

    Plasmid

    #208576

    Purpose

    Empty NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    Empty response element
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and miniP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-puro-Flag-mMcl-1

    Plasmid

    #32982

    Depositor

    Joseph Opferman

    Article

    Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

    Insert

    Mcl-1 (Mcl1 Mouse)
    

    Use

    Retroviral

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pGL3-PUb

    Plasmid

    #52891

    Purpose

    Aedes aegypti polyubiquitin promoter driving firefly luciferase

    Depositor

    Zach Adelman

    Article

    Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

    Insert

    polyubiquitin promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Ae aegypti polyubiquitin

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2xMARS-nGFP

    Plasmid

    #205235

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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