We narrowed to 148,842 results for: ins;

1. EGFP-Cyclin D2

   Plasmid

   #215051

   Purpose

   Expresses EGFP-Cyclin D2 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SS-Cyclin D1

   Plasmid

   #215113

   Purpose

   Expresses SS-Cyclin D1 in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Tags

   SS (STREP-STREP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-POLE1

   Plasmid

   #215140

   Purpose

   Expresses mCherry-POLE1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   mCherry_FWR

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acid 80-86 and 211-217 …

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting

   Plasmid

   #92141

   Purpose

   Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   osTir1
   

   Use

   Mouse Targeting

   Tags

   V5 tag

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR4_miniFXN_7

   Plasmid

   #234360

   Purpose

   Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.

   Depositor

   Marek Napierala

   Article

   Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

   Insert

   FXN (FXN Human)
   

   Use

   Cloning

   Tags

   Flag Tag

   Mutation

   There is a partial deletion in the 5'UTR of …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. hsp70l: hAPOB48-mCherry.

   Plasmid

   #140639

   Purpose

   Heat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.

   Depositor

   Steven Farber

   Article

   Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

   Inserts

   hsp70l (hsp70l Zebrafish)
   APOB (APOB Human)
   

   Use

   Zebrafish expression

   Tags

   mCherry

   Mutation

   APOB48: amino acid 1-2180

   Promoter

   hsp70l

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Cyclin B1

   Plasmid

   #215049

   Purpose

   Expresses EGFP-Cyclin B1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCNB1 (CCNB1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

   Plasmid

   #115694

   Purpose

   All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Inserts

   NmeCas9
   Nme-sgRNA
   

   Use

   CRISPR

   Tags

   NLS and NLS, HA

   Expression

   Insect and Mammalian

   Mutation

   None and fusion of repeat/tracrRNA to make a sgRNA

   Promoter

   EF-1 alpha and U6

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-APEX2

   Plasmid

   #215127

   Purpose

   Expresses mCherry-APEX2 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   APEX2 (APEX2 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

    Plasmid

    #114078

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLgw Cbx1-V5-EcoDam

    Plasmid

    #59211

    Purpose

    Mammalian DamID positive control lentiviral plasmid. Expresses Cbx1-V5-Dam.

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Insert

    Cbx1 (Cbx1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam and V5

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter and heat shock minima…

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. STT3A pEGFP-N2 (1051)

    Plasmid

    #62025

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. EXO1B-AcGFP

    Plasmid

    #215125

    Purpose

    Expresses EXO1B-AcGFP in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    EXO1B (EXO1 Human)
    

    Use

    Retroviral

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCerulean-PCNA-HygroR

    Plasmid

    #215064

    Purpose

    Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-PA-Rac1-I539E

    Plasmid

    #22026

    Purpose

    Expression of photoactivatable Rac1 ('lit' state mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
    

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains 'lit state' mutatio…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. mCerulean-PCNA-PuroR

    Plasmid

    #215063

    Purpose

    Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUSEss-CHIg-mM-Asn209Asp_M18

    Plasmid

    #91781

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Mutation

    Asn209 changed to Asp (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. mKO2-CDK4

    Plasmid

    #215065

    Purpose

    Expresses mKO2-CDK4 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Retroviral

    Tags

    mKO2

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid

    #194190

    Purpose

    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    5 mutated KLF binding sites (positions 449,548,73…

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-pSMANTIS_1kb_KLF2mut 899

    Plasmid

    #194193

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    mutated KLF binding sites (position 899)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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