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SUMO-Twinkle 43-684

Plasmid

#205056

Purpose

TWNK protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

TWNK (TWNK Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks amino acid from 1-42

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO-Twinkle 360-684

Plasmid

#205055

Purpose

TWNK protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

TWNK (TWNK Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks N terminal amino acid from 1-359

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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PTPRA_C433S-SHC

Plasmid

#139612

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA_C433S (PTPRA Human)

Use

Tags

StrepIII-HA

Expression

Mammalian

Mutation

C433S

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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PTPRA_Y789F-SHC

Plasmid

#139614

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA_Y789F (PTPRA Human)

Use

Tags

StrepIII-HA

Expression

Mammalian

Mutation

Y789F

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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PTPRA_deltaD2-GFP fusion

Plasmid

#139641

Purpose

GFP-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA_deltaD2 (PTPRA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

D2 domain deletion

Promoter

CMV

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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PTPRA_deltaD1-GFP fusion

Plasmid

#139640

Purpose

GFP-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA_deltaD1 (PTPRA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

D1 domain deletion

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

Plasmid

#128764

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #3 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

Plasmid

#128763

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #2 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PRRP-Tango

Plasmid

#66480

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

PRRP (PRLH Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEA01

Plasmid

#170766

Purpose

Utilisation of pSAM2 excision tool in Amycolatopsis spp

Depositor

Jean-Luc Pernodet

Article

Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

Inserts

xis, int
oriT
pac

Use

Tags

Expression

Bacterial

Mutation

Promoter

trcp

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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EF.GFP

Plasmid

#17616

Depositor

Linzhao Cheng

Article

Cui et al Blood. 2002 Jan 15. 99(2):399-408.

Insert

EF-1a promoter (EEF1A1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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PTPRA-MAC

Plasmid

#139632

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA (PTPRA Human)

Use

Tags

StrepIII-HA-BirA*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Myc-NLRP3(R260W)

Plasmid

#73956

Purpose

Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

Depositor

Christian Stehlik

Article

Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

R260W

Promoter

CMV iE

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
FPR3-Tango

Plasmid

#66285

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

FPR3 (FPR3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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PLRG1

Plasmid

#155827

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PLRG1 (PLRG1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SUMO-Twinkle 43-372

Plasmid

#205054

Purpose

TWNK protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

TWNK (TWNK Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks Mitochondrial localization signal and C ter…

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PDGFRL gRNA (BRDN0001145943)

Plasmid

#77697

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDGFRL

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDGFRL (PDGFRL Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PDGFRL gRNA (BRDN0001147851)

Plasmid

#77696

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDGFRL

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDGFRL (PDGFRL Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PDGFRL gRNA (BRDN0001147755)

Plasmid

#77698

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDGFRL

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDGFRL (PDGFRL Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge