We narrowed to 9,167 results for: Pol;

1. pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)

   Plasmid

   #156162

   Purpose

   Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   THBS1 with deletion of amino acid residues 383-548

   Promoter

   CMV

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET empty polycistronic destination vector (2Z)

   Plasmid

   #29776

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-Flag-Polß(WT)-IRES-puro

   Plasmid

   #128653

   Purpose

   mammalian expression of Flag-Polß(WT) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   CMV

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

   Plasmid

   #163545

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Polymerase expression - pCMV-ePhi29 (LM2802)

   Plasmid

   #208967

   Purpose

   Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

   Plasmid

   #124542

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41 C55G

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDH-EF1-FHC-POLQ-S1977P

   Plasmid

   #64876

   Purpose

   lentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   S1977P, mimicking the chaos1 mutation

   Promoter

   EF1alpha

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124543

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19-TgKI-MCS-mLanYFP-polyA

    Plasmid

    #174021

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    mLanYFP
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YIp128-P30-His-pol30(K164R)

    Plasmid

    #99544

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K164R

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-ELYS-emerin-EGFP-polyA

    Plasmid

    #59745

    Purpose

    Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    ELYS-emerin-EGFP
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)

    Plasmid

    #156163

    Purpose

    Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Truncated THBS1 (amino acid residues 1-954)

    Promoter

    CMV

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124546

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. attB-mCh-Dnmt3b1-Poly(A)-PuroR

    Plasmid

    #65556

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 1

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pspCas9-POLI-ST2-com vector

    Plasmid

    #176234

    Purpose

    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXS-IRES-Blast POLE1 N-ISCmut

    Plasmid

    #160803

    Purpose

    Express N-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 N-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

    Plasmid

    #159487

    Purpose

    Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-2_PolyU(1)
    

    Expression

    Mammalian

    Promoter

    U6-27

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. attB-mCh-Dnmt3b6-Poly(A)-NeoR

    Plasmid

    #65554

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 6

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-Flag-Polß(WT)-IRES-Neo

    Plasmid

    #128663

    Purpose

    mammalian expression of Flag-Polß(WT) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid

    #192585

    Purpose

    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    Lentiviral

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    Ef1-alpha

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMC-Z-SpCas9-gRNA-repeat-polyT-11

    Plasmid

    #197781

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    gRNA repeat for U6 sgRNA assembly
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. C413-E22: attB4-polyhedrinp>-attB1r

    Plasmid

    #162922

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)

    Plasmid

    #155195

    Purpose

    Expresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    THBS1 with deletion of amino acid residues 276-315

    Promoter

    CMV

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXS-IRES-Blast POLE1 C-ISCmut

    Plasmid

    #160804

    Purpose

    Express C-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 C-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KZ401: pMVP (L3-L2) P2A-Neo + polyA

    Plasmid

    #121781

    Purpose

    pMVP L3-L2 entry plasmid, contains Neo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Neo + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KZ601: pMVP (L3-L2) P2A-Zeo + polyA

    Plasmid

    #121783

    Purpose

    pMVP L3-L2 entry plasmid, contains Zeo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Zeo + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124541

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124539

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge