We narrowed to 1,239 results for: membrane gfp

1. pCMV_SEPT9_i3 NCmut-msfGFP

   Plasmid

   #180325

   Purpose

   mammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i3 I263D, M270D

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-DIO-EGFP-CAAX

   Plasmid

   #208685

   Purpose

   Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   membrane-localized enhanced green fluorescent protein EGFP-CAAX
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-mGFP

   Plasmid

   #14757

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Has Service

   Cloning Grade DNA

   Insert

   membrane bound form of EGFP
   

   Use

   Tags

   Palmitoylation signal

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. UVR8-GFPCaaX

   Plasmid

   #49805

   Purpose

   expresses UVR8 fused to GFP with a prenylation motif for membrane targeting

   Depositor

   Matthew Kennedy

   Article

   Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

   Insert

   UVR8-GFPCaax (UVR8 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mPA-GFP-CD151-7

   Plasmid

   #57125

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   CD151 (CD151 Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. mPA-GFP-Caveolin-C-10

   Plasmid

   #57123

   Purpose

   Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Caveolin (CAV1 Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. mPA-GFP-FYN-N-10

   Plasmid

   #57135

   Purpose

   Localization: Membrane Associated Kinase, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   FYN (FYN Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. Venus-BclXL-ActA-pEGFP-C1

   Plasmid

   #177410

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

   Depositor

   David Andrews

   Insert

   Bcl-XL-ActA, BclX-acta
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Venus-BclXL-Cb5-pEGFP-C1

   Plasmid

   #177415

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).

   Depositor

   David Andrews

   Insert

   Bcl-XL-Cb5, BclX-cb5
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mPA-GFP-Farnesyl-5

    Plasmid

    #57133

    Purpose

    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. CMV-SNAP-hCB1_IRES_EGFP

    Plasmid

    #223505

    Purpose

    Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

    Depositor

    James Frank

    Insert

    SNAP-tag-CNR1 and EGFP (CNR1 Synthetic, Human)
    

    Use

    Tags

    SNAP-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ath5:GFP-CAAX

    Plasmid

    #105958

    Purpose

    transgenesis, EGFP membrane

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    CAAX
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3

    Plasmid

    #198451

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCerulean3-BclXL-ActA-pEGFP-C1

    Plasmid

    #177412

    Purpose

    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-XL-ActA, BclX-acta
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCerulean3-BclXL-Cb5-pEGFP-C1

    Plasmid

    #177414

    Purpose

    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-XL-Cb5, BclX-cb5
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Venus-Bcl2-ActA-pEGFP-C1

    Plasmid

    #177419

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Venus-Bcl2-cb5-pEGFP-C1

    Plasmid

    #177422

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GPI-mEGFP(2x)

    Plasmid

    #197911

    Purpose

    Homo-dimer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

    Insert

    glycosylphosphatidylinositol-mEGFP-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PMT-mEGFP

    Plasmid

    #203777

    Purpose

    Brightness calibration

    Depositor

    Thorsten Wohland

    Article

    Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

    Insert

    PMT-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PMT-mEGFP_2

    Plasmid

    #203778

    Purpose

    Brightness calibration

    Depositor

    Thorsten Wohland

    Article

    Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

    Insert

    PMT-mEGFP dimer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR SFFV GLP1R(S301A)-EGFP

    Plasmid

    #158123

    Purpose

    Lentiviral plasmid for expression of GLP1R(S301)-EGFP fusion with SFFV promoter.

    Depositor

    Neal Devaraj

    Insert

    GLP1R (GLP1R )
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Serine 301 changed to alanine to enhance membrane…

    Promoter

    SFFV

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP Cav2.2 human HAgHA-plus exon 18a - Short-Cterm - Arginine -in pcDNA3

    Plasmid

    #198915

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    snp rs2278973 Arginine

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP Cav2.2 HA L702P pcDNA3

    Plasmid

    #206056

    Purpose

    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional

    Depositor

    Annette Dolphin

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L702P

    Promoter

    CMV

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP Cav2.2 HA D665A pcDNA3

    Plasmid

    #206113

    Purpose

    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional

    Depositor

    Annette Dolphin

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    D665A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Venus-7aa-Cb5-pEGFP-C1

    Plasmid

    #166764

    Purpose

    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5a Rat)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP Cav2.2 HA L702 P pCAGGS

    Plasmid

    #206055

    Purpose

    expression of rabbit Cav2.2 with N-terminal GFP tag and exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional

    Depositor

    Annette Dolphin

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L702P

    Promoter

    CMV/B-actin

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCerulean3-Bcl2-ActA-pEGFP-C1

    Plasmid

    #177418

    Purpose

    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCerulean3-Bcl2-cb5-pEGFP-C1

    Plasmid

    #177421

    Purpose

    Express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. myr-palm-mCherry2-mEGFP

    Plasmid

    #182859

    Purpose

    Hetero-dimer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    myr-palm-mCherry2-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. myr-palm-mEYFP-(L)-mEGFP

    Plasmid

    #182852

    Purpose

    Hetero-dimer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    myr-palm-mEYFP-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP

    Plasmid

    #180311

    Purpose

    bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    SEPT2 F20D, V27D

    Promoter

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAGGS-myrGFP

    Plasmid

    #115502

    Purpose

    expression of myristoylated, plasma membrane targeted GFP

    Depositor

    Thomas Biederer

    Article

    Stagi et al Proc Natl Acad Sci U S A. 2010 Apr 20;10

    Insert

    myristoylated GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

33. GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3

    Plasmid

    #198914

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    exon 18a deleted, exon 19 starts 3 nucleotides ea…

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid

    #177427

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycines 153 and 154 mutated to alanine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid

    #177428

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycine 154 replaced with STOP codon

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP Cav2.2 human -HAgHA-minus exon 18a -Short-Cterm - Arg -in pcDNA3

    Plasmid

    #198916

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    exon 18a deleted, exon 19 starts 3 nucleotides ea…

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GFP-OMP25

    Plasmid

    #141150

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    OMP25 c terminus from addgene plasmid #69598

    Promoter

    CMV

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Tet-myr-GFP

    Plasmid

    #83468

    Purpose

    Lentiviral vector for doxycycline-inducible expression of membrane targeted-GFP in mammalian cells

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Myristoylated GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline-inducible promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. myr-palm_mCherry2-mEGFP

    Plasmid

    #127816

    Purpose

    Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mCherry2-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. myr-palm_mCherry-mEGFP

    Plasmid

    #127815

    Purpose

    Hetero-dimer mCherry-mEGFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mCherry-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge