We narrowed to 2,904 results for: BFP

1. pKLV2-U6gRNA5(SOX2_v32_7-5)-PGKpuroBFP-W

   Plasmid

   #211987

   Purpose

   Express gRNA against SOX2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting SOX2 (SOX2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Str-KDEL_flGalT-SBP-tagBFP

   Plasmid

   #65274

   Purpose

   synchronize trafficking of GalT from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   galactosidase
   

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2410-Tier1-PhCMV-Myr_mTagBFP2

   Plasmid

   #169535

   Purpose

   Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPRISM-TagRFP-cmlc2-TagBFP

   Plasmid

   #117768

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FKBP12-CB2x2-mTagBFP2-HAx2

   Plasmid

   #207146

   Purpose

   Tandem calcium-buffer with rapalog dimerizer

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   Calbindin-2 (CALB2 Human)
   

   Tags

   FKBP12, HA, and mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W

   Plasmid

   #208554

   Purpose

   Lentiviral vector expressing gRNA targeting human BCL11B-E1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   BCL11B-E1(14) (BCL11B Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Thy1.2-NLS_HA_Dre-P2A-BFP

   Plasmid

   #51275

   Purpose

   Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS_HA_Dre-P2A-BFP
   

   Promoter

   Thy1.2

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

   Plasmid

   #52478

   Purpose

   Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

   Depositor

   Sebastian Seung

   Article

   Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

   Inserts

   EGFP
   nuclear mTagBFP
   Synaptophysin-TagRFP-T
   

   Use

   Rabies virus

   Tags

   TagRFP-T

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

   Plasmid

   #105037

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nprl2 (Nprl2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

    Plasmid

    #105038

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-TRE-mTagBFP2-WPRE

    Plasmid

    #193332

    Purpose

    Expresses mTagBFP2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mTagBFP2
    

    Expression

    Mammalian

    Mutation

    A XhoI site in the coding sequence is silenced

    Promoter

    TRE

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dTomato_LD 0_CS G_tTA-BFP

    Plasmid

    #58871

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Str-KDEL_ManII-SBP-tagBFP

    Plasmid

    #65254

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry_LD 0_CS G_tTA-BFP

    Plasmid

    #58856

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC-U63-tgRNA-nlsBFP

    Plasmid

    #169029

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

    Plasmid

    #105023

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

    Plasmid

    #105024

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

    Plasmid

    #105025

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

    Plasmid

    #105026

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-Trex2-bpA-EF1BFP

    Plasmid

    #111144

    Purpose

    Expression of mouse Trex2 and BFP reporter in mammalian cells

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Inserts

    mouse Trex2 coding region (Trex2 Mouse)
    BFP (Rnf112 )
    

    Expression

    Mammalian

    Promoter

    CAG and EF1

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPRISM-Stop-cmlc2-TagBFP

    Plasmid

    #117774

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    3x3 Stop sequence
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ptagBFP-LacI-NLS-VP16

    Plasmid

    #103837

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-LacI-NLS-VP16
    

    Expression

    Mammalian

    Promoter

    SV2

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPRISM-Fuse-gcry-TagBFP

    Plasmid

    #117783

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    mRFP
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-FKBP-mTagBFP2-MCS

    Plasmid

    #178864

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mTagBFP2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    FKBP-mTagBFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPRISM-Cre-gcry-TagBFP

    Plasmid

    #117789

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    Cre
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)

    Plasmid

    #128171

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHR Src deltaSH2/3-TagBFP

    Plasmid

    #171033

    Purpose

    TagBFP-tagged Src variant lacking SH2 and SH3 domains.

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

    Insert

    Src
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPRISM-Cre-cmlc2-TagBFP

    Plasmid

    #117792

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    Cre
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98976

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(N863A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLIX403-EYFP-ccdB-EBFP2

    Plasmid

    #158553

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

    Plasmid

    #163138

    Purpose

    Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MSCV-Scara3-IRES-BFP

    Plasmid

    #117265

    Purpose

    Retroviral overexpression of Scara3

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    Scara3 (Scara3 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    LTR

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

    Plasmid

    #84388

    Purpose

    Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-Char-BFP2-TSERex

    Plasmid

    #124792

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Char-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CD4_LD 0_CS G_tTA-BFP

    Plasmid

    #58869

    Purpose

    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W

    Plasmid

    #105019

    Purpose

    Lentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Vps39 (Vps39 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ptagBFP-TetR-NLS-VP16

    Plasmid

    #103835

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-TetR-NLS-VP16 (tetR )
    

    Expression

    Mammalian

    Promoter

    SV2

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

    Plasmid

    #105027

    Purpose

    Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTagBFP2-C1-AKAP1N31-FKBP-PI-PLCN187

    Plasmid

    #139309

    Purpose

    N-terminal half of split PI-PLC targeted to mitochondria

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Mus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
    

    Expression

    Mammalian

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKLV2-U6gRNA5(gKAT7-5)-PGKpuro2ABFP-W

    Plasmid

    #159287

    Purpose

    Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. 5)

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    guide RNA targeting KAT7 (ID. 5) (KAT7 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBlastR-12xLexAOp-EBFP2-MiniWhite

    Plasmid

    #165918

    Purpose

    Blasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mCherry_LD 0_CS M_tTA-BFP

    Plasmid

    #58874

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mCherry_LD 0_CS A_tTA-BFP

    Plasmid

    #58873

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRVdG-mCherry-N-P-M-nucBFP-EGFP-L

    Plasmid

    #52484

    Purpose

    Expresses mCherry, nuclear mTagBFP and EGFP

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

    Inserts

    mCherry
    nuclear mTagBFP
    EGFP
    

    Use

    Rabies virus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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45. pLenti-EBFP-VAV1-W495L

    Plasmid

    #242839

    Purpose

    Encodes human VAV1 and N-terminal EBFP2 tag

    Depositor

    Cheng-han Yu

    Article

    Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

    Insert

    VAV1 (VAV1 Human)
    

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    W495L

    Available Since

    Oct. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLenti-EBFP2-VAV1-E201A

    Plasmid

    #242838

    Purpose

    Encodes human VAV1 and N-terminal EBFP2 tag

    Depositor

    Cheng-han Yu

    Article

    Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

    Insert

    VAV1 (VAV1 Human)
    

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    E201A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. VEE-moxBFP-IRES-PuroR

    Plasmid

    #242413

    Purpose

    Spectral unmixing: moxBFP only

    Depositor

    Ewan St. John Smith

    Article

    Lim et al bioRxiv 2024.09.24.614636

    Inserts

    moxBFP
    IRES-PuroR
    

    Use

    Self-amplifying rna

    Expression

    Mammalian

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCMV mTagBFP2-2A-CHMP2B-Q165X

    Plasmid

    #232001

    Purpose

    Bicistronic expression of CHMP2B Q165X mutant along with an mTagBFP2 transfection marker, connected via 2A sequences.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Expression

    Mammalian

    Mutation

    Q165X

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pTRIP-SFFV-tagBFP-2A-IE1

    Plasmid

    #235541

    Purpose

    To express tagBFP-2A-IE1

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    IE1
    

    Use

    Lentiviral

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pTRIP-SFFV-tagBFP-2A-coICP0

    Plasmid

    #235536

    Purpose

    To express tagBFP-2A-coICP0 (co = codon-optimized)

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    ICP0
    

    Use

    Lentiviral

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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