We narrowed to 3,084 results for: PPL

1. pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

   Plasmid

   #196250

   Purpose

   Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL1-GlpT-scGFP-attL2
   

   Expression

   Bacterial

   Promoter

   J23119 promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET PPL His6 MBP LIC cloning vector (2K-T)

   Plasmid

   #37183

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6, MBP, and PPL

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

   Plasmid

   #217970

   Purpose

   Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

   Depositor

   Robert Piper

   Article

   Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

   Insert

   2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

   Plasmid

   #204724

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

   Plasmid

   #217971

   Purpose

   Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.

   Depositor

   Robert Piper

   Article

   Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

   Insert

   2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
   

   Expression

   Mammalian

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

   Plasmid

   #216694

   Purpose

   Synchronize trafficking of GPI-AP from the ER (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   SBP-mApple-FIREtag-GPI
   

   Tags

   FIREtag, IL2 signal sequence, Streptavidin Bindin…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

   Plasmid

   #216695

   Purpose

   Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
   

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

   Plasmid

   #105842

   Purpose

   Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   ISL LIM Homeobox 1 (ISL1 Human)
   LIM Homeobox 3 (LHX3 Human)
   Optimized mApple
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBPcyto-TEV-BseRI

   Plasmid

   #157739

   Purpose

   empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Tags

   His10 + MBP (cytoplasmic expression; no signal se…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH_35S/mEGFP

    Plasmid

    #135321

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB_35S/mEGFP

    Plasmid

    #135320

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN_35S/mEGFP

    Plasmid

    #132565

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GS2.1/EC

    Plasmid

    #132568

    Purpose

    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    egg cell promoter, Cas9-mApple, pds3 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    A. thaliana egg cell promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSII42K

    Plasmid

    #194523

    Purpose

    High copy vector backbone with G418 selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSII41K

    Plasmid

    #194522

    Purpose

    Low copy vector backbone with G418 selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSII41N

    Plasmid

    #194524

    Purpose

    Low copy vector backbone with nourseothricin selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSII42N

    Plasmid

    #194525

    Purpose

    High copy vector backbone with nourseothricin selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSII41H

    Plasmid

    #194526

    Purpose

    Low copy vector backbone with hygromycin B selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSII42H

    Plasmid

    #194527

    Purpose

    High copy vector backbone with hygromycin B selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSII42H-TDH3pr

    Plasmid

    #194533

    Purpose

    High copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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