We narrowed to 14,145 results for: TIM

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pRS413-TEF1pr-ERG6-FAPoptim

Plasmid

#221121

Purpose

FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ERG6-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-PMA1-FAPoptim

Plasmid

#221122

Purpose

FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

PMA1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-RPA34-FAPoptim

Plasmid

#221123

Purpose

FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

RPA34-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC7-FAPoptim

Plasmid

#221124

Purpose

FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC7-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-VPH1-FAPoptim

Plasmid

#221125

Purpose

FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

VPH1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-EBFP2-OptoSTIM1(CRY2clust)

Plasmid

#184716

Purpose

OptoSTIM1(CRY2clust)

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

OptoSTIM1

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

Plasmid

#200639

Purpose

Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

STIM1 (STIM1 Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1optim_1676-2193_U

Plasmid

#147729

Purpose

Bacterial Expression of CtNot1optim_1676-2193

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

CtNot1optim_1676-2193

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-DmAgo1_572-984-optim_K

Plasmid

#146728

Purpose

Bacterial Expression of DmAgo1_572-984-optim

Depositor

Elisa Izaurralde

Insert

DmAgo1_572-984-optim (AGO1 Fly)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtTIM21(1-50aa)-mCherry

Plasmid

#194047

Purpose

Transient expression of AtTIM21-mCherry in plant cell (Mitochondria)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

mCherry

Tags

AtTIM21 1–50aa

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1 (BTLA ITIM)-mGFP

Plasmid

#180817

Purpose

Expression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

PD-1 (BTLA ITIM) (PDCD1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…

Promoter

SFFV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-BTLA (PD-1 ITIM)-mGFP

Plasmid

#180818

Purpose

Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

BTLA (PD-1 ITIM) (BTLA Human)

Use

Lentiviral

Tags

mEGFP

Mutation

replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…

Promoter

SFFV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-optimized E6AP F727D

Plasmid

#165101

Purpose

ecoli optimized expression of a GST fusion of Human E6AP F727D

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

Insert

E6AP F727D (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-SIRPT1-Latimeria chalumnae

Plasmid

#75324

Purpose

GST fusion Latimeria chalumnae Sacsin 94-1368

Depositor

Kalle Gehring

Insert

Sacsin Latimeria chalumnae 94-1368

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET-15b_T. maritima His-tRNase Z

Plasmid

#242436

Purpose

Expresses T. maritima tRNase Z in E. coli.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

tRNase Z

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW PuroR-P2A-COX8ASig-Timer

Plasmid

#239559

Purpose

Expression and mitohcondrial localization of Timer

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

Timer

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDae069 (eMutaT7[PmCDA1] with optimized RBS)

Plasmid

#187621

Purpose

Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

PmCDA1-T7RNAP

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-NLRP1 codon-optimized

Plasmid

#166833

Purpose

Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Promoter

CMV,&Rhamnose

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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