We narrowed to 1,635 results for: TYR

1. pcDNA3.1-AXL-V5/HIS

   Plasmid

   #200117

   Purpose

   Expression of human AXL receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human AXL receptor tyrosine kinase (AXL Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-DDR2-V5/HIS

   Plasmid

   #201101

   Purpose

   expression of human DDR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human DDR2 receptor tyrosine kinase, full length, wildtype (DDR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-DDK_WT POLI

   Plasmid

   #131228

   Purpose

   Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Coding sequence has been codon optimised for expr…

   Promoter

   CMV enhancer + CMV promoter

   Available since

   Dec. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV5 Flag p38 alpha(agf)

   Plasmid

   #20787

   Depositor

   Roger Davis

   Article

   Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

   Insert

   pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   replaced Thr180 and Tyr182 with Ala and Phe, resp…

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

5. Flag‐mTim3 (T2 truncation)

   Plasmid

   #49211

   Purpose

   expresses Flag tagged mouse Tim3 (T2 truncation)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 T2 truncation (Havcr2 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   T2 truncation ( lacks all but one tyrosine, which…

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-His6-SUMO-cSrc-SH2-AviTag

   Plasmid

   #214230

   Purpose

   Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

   Depositor

   Neel Shah

   Article

   Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

   Insert

   cSrc kinase SH2 domain (SRC Human)
   

   Use

   Tags

   6xHis, AviTag, and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

   Plasmid

   #70085

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

   Promoter

   SV40

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-ZIP(FF)

   Plasmid

   #27135

   Depositor

   Ron Vale

   Article

   Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

   Insert

   CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   eGFP and mCherry

   Expression

   Mammalian

   Mutation

   Tyrosines in ITAMS 1-3 mutated to phenylalanines …

   Promoter

   Available since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7-EF-EYFP-YAP1_Y357F-PolyA

   Plasmid

   #112287

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoform

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Tyrosine 357 to Phenyalanine corresponding to tyr…

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228788

    Purpose

    EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. NGFR 210Y177

    Plasmid

    #27487

    Depositor

    Warren Pear

    Article

    He et al Blood. 2002 Apr 15;99(8):2957-68.

    Insert

    bcr/abl (P210) (ABL1, BCR Human)
    

    Use

    Retroviral

    Tags

    IRES and NGFR

    Expression

    Mammalian

    Mutation

    Tyrosine 177 mutated to Phenylalanine (Y177F)

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228787

    Purpose

    EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF2-EFGR-hMer

    Plasmid

    #225996

    Purpose

    Expresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTK

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

    Insert

    human EGFR N-Terminal human MerTK C-Terminal Fusion (EGFR, MERTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF2-EFGR-hAxl

    Plasmid

    #225997

    Purpose

    Expresses a chimeric contruct having N-terminal human EGFR and C-terminal human Axl

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

    Insert

    human EGFR N-Terminal human Axl C-Terminal Fusion (EGFR, AXL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZE21/UBP1/ClpS_V65I

    Plasmid

    #98567

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains the V65I mutation that increases discrim…

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TH1148-pOCC177-FUS(Nhe)-PLD_YtoF

    Plasmid

    #221887

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    In PLD all Tyr (Y) changed to Phe (F)

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-2B-Nkx2-5YRD Y-A

    Plasmid

    #98611

    Purpose

    Gateway entry vector for NKX2-5 YRD Y-A

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 YRD Y-A (Nkx2-5 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Substitutions of 7 tyr into Ala residues.Y233A; Y…

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL0_153 [pCB102 ORI]

    Plasmid

    #210675

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Insert

    pCB102 ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pY125 (pcDNA3.1-huBfCpf1)

    Plasmid

    #92301

    Purpose

    Expression of humanized BfCpf1

    Depositor

    Feng Zhang

    Insert

    huBfCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pY124 (pcDNA3.1-huBsCpf1)

    Plasmid

    #92300

    Purpose

    Expression of humanized BsCpf1

    Depositor

    Feng Zhang

    Insert

    huBsCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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