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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 945 results for: dcas9, grna

Showing: 281 - 300 of 945 results

  5. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid
    #188899
    Purpose
    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Zim3-dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion
    Expression
    Mutation
    Promoter
    SFFV
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188899-plasmid-map-sequence-id-374831

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  6. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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  7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available Since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    118155-plasmid-map-sequence-id-243704

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  13. nAPEX2-V5-zdCas9n-3x-FLAG-GSG-linker-P2A-mNeongreen

    Plasmid
    #241191
    Purpose
    Middle entry vector for mini-Golden subcloning platform; 3x-FLAG; mNeongreen
    Depositor
    Junsu Kang
    Insert
    3x-FLAG; mNeongreen
    Use
    Mini-golden
    Tags
    Expression
    Mutation
    TCGC linker
    Promoter
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241191-plasmid-map-sequence-id-482037

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  14. 3x-FLAG-nzdCas9-V5-APEX2n-GSG-linker-P2A-mNeongreen

    Plasmid
    #241190
    Purpose
    Middle entry vector for mini-Golden subcloning platform; V5-APEX2n; mNeongreen
    Depositor
    Junsu Kang
    Insert
    V5-APEX2n; mNeongreen
    Use
    Mini-golden
    Tags
    Expression
    Mutation
    TCGC linker
    Promoter
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241190-plasmid-map-sequence-id-482036

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Showing: 281 - 300 of 945 results