We narrowed to 1,027 results for: gibson assembly

1. pST39-Mega'-deltaNF

   Plasmid

   #216909

   Purpose

   Mega' with knockout of GvpN and GvpF

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpN and GvpF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST39-Mega'-deltaRU

   Plasmid

   #216908

   Purpose

   Mega' with knockout of GvpR and GvpU

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpR and GvpU
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pST39-Mega'-deltaRT

   Plasmid

   #216907

   Purpose

   Mega' with knockout of GvpR and GvpT

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpR and GvpT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pST39-Mega'-deltaAll

   Plasmid

   #216878

   Purpose

   Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but all gas vesicle proteins have been deleted.

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pST39-Mega'-deltaJT

   Plasmid

   #216942

   Purpose

   Mega' with knockout of GvpJ and GvpT

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpJ and GvpT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pST39-Mega'-deltaST

   Plasmid

   #216937

   Purpose

   Mega' with knockout of GvpS and GvpT

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpS and GvpT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pST39-Mega'-deltaSJ

   Plasmid

   #216936

   Purpose

   Mega' with knockout of GvpS and GvpJ

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpS and GvpJ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pST39-Mega'-deltaG

   Plasmid

   #216883

   Purpose

   Mega' with knockout of GvpG

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pST39-Mega'-deltaLJ

   Plasmid

   #216932

   Purpose

   Mega' with knockout of GvpL and GvpJ

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Mega' with knockout of GvpL and GvpJ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pST39-Mega'-deltaLK

    Plasmid

    #216931

    Purpose

    Mega' with knockout of GvpL and GvpK

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpL and GvpK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pST39-Mega'-deltaGU

    Plasmid

    #216929

    Purpose

    Mega' with knockout of GvpG and GvpU

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpG and GvpU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pST39-Mega'-deltaGT

    Plasmid

    #216928

    Purpose

    Mega' with knockout of GvpG and GvpT

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpG and GvpT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pST39-Mega'-deltaGK

    Plasmid

    #216926

    Purpose

    Mega' with knockout of GvpG and GvpK

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpG and GvpK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pST39-Mega'-deltaGJ

    Plasmid

    #216927

    Purpose

    Mega' with knockout of GvpG and GvpJ

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpG and GvpJ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST39-Mega'-deltaTU

    Plasmid

    #216944

    Purpose

    Mega' with knockout of GvpT and GvpU

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpT and GvpU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-39S Light chain

    Plasmid

    #216297

    Purpose

    Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    39S Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-MF3958 Light chain

    Plasmid

    #216304

    Purpose

    Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    MF3958 Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-SnoopTag-AffiHER2-SpyTag

    Plasmid

    #216280

    Purpose

    Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-AffiHER2-SpyTag
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

    Plasmid

    #185679

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-2xHaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

    Plasmid

    #137110

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    MBP-CS(TEV)-StrepTag-II-AI(TVMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge