We narrowed to 143,240 results for: ins;

1. pCD5-PR8-TEV-sfGFP-twinStrep

   Plasmid

   #157933

   Purpose

   Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

   Insert

   A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
   

   Use

   Tags

   sfGFP & TwinStrep

   Expression

   Mammalian

   Mutation

   E190D

   Promoter

   CMV

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

   Plasmid

   #159283

   Purpose

   A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Inserts

   Human INS sgRNA
   Human INS guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fusion of Zika NS2B-NS3 proteins

   Plasmid

   #204815

   Purpose

   Bacterial expression of a fusion of Zika NS2B-NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Zika NS2B-NS3 fusion (orf1ab Zika)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. Fusion of Dengue2 NS2B-NS3 proteins

   Plasmid

   #204792

   Purpose

   Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Dengue2 NS2B-NS3 fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pMSCV-IRES-GFP/CALR ins5

   Plasmid

   #214701

   Purpose

   Mammalian expression of human CALR ins5

   Depositor

   Norio Komatsu

   Article

   Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

   Insert

   CALR ins5 (CALR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   MSCV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEA1-Puro-HEK3 CTT ins

   Plasmid

   #171996

   Purpose

   Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgRNA(MS2)_zeo insert c-MYC

   Plasmid

   #164636

   Purpose

   sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

   Depositor

   Günter Schneider

   Insert

   target sequence for c-Myc activation (MYC Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK5 FLAG DEPTOR (DEP domains)

   Plasmid

   #21700

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 13. ():.

   Insert

   DEPTOR DEP domains (DEPTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   DEP domains only

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1 GIL-11 myc TEV TwinStrep

   Plasmid

   #199627

   Purpose

   Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

   Depositor

   Jurgen Scheller

   Article

   Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

   Insert

   GIL-11
   

   Use

   Tags

   TwinStrep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid

    #124660

    Purpose

    reporter protein which emits bright blue fluorescence

    Depositor

    Yutaka Kuroda

    Article

    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

    Insert

    Gaussia luciferase-C9D-TGinsert
    

    Use

    Tags

    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

    Expression

    Bacterial

    Mutation

    changed glutamic acid 100 to alanine, and glycine…

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

    Plasmid

    #139770

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal TwinStrep tag
    

    Use

    Tags

    3C - TwinStrep tag

    Expression

    Mutation

    Promoter

    PH

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AtS/MAR10 insulator sequence (GB3458)

    Plasmid

    #160638

    Purpose

    pEGB3a1 AtS/MAR10

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    AtS/MAR10 insulator sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMS4 (Empty insertion vector with SEC)

    Plasmid

    #154837

    Purpose

    Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    SEC
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Foxd3 CreERT2 RMCE Insertion Vector

    Plasmid

    #37272

    Depositor

    Patricia Labosky

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    Mouse Targeting; Insertion vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pOPINS-HOIP NZF1 (351-379)

    Plasmid

    #235655

    Purpose

    Bacterial expression of human HOIP NZF1 (351-379)

    Depositor

    David Komander

    Article

    Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

    Insert

    HOIP NZF1 (RNF31 Human)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 351-379 only

    Promoter

    T7

    Available since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

    Plasmid

    #200877

    Purpose

    ZooTsy_TnsF expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    ZooTsy_TnsF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TnsF-Y584F

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HEK4-53bp-tag-insertion-pegRNA

    Plasmid

    #227673

    Purpose

    PegRNA for 53 bp insertion

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    SpCas9 sgRNA scaffold (EPHA3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2-HsProm1s1(W795R)-TwinStrep-His

    Plasmid

    #210817

    Purpose

    Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Mammalian

    Mutation

    Changed Trp-795 to Arg

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-IRES-mOrange/CALR ins5

    Plasmid

    #214769

    Purpose

    Mammalian expression of human CALR ins5

    Depositor

    Norio Komatsu

    Article

    De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

    Insert

    human CALR ins5 (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Fusion of Dengue2 NS2B-NS3 proteins

    Plasmid

    #204793

    Purpose

    Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue2 NS2B-NS3 fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge