We narrowed to 148,387 results for: ins;

1. INS-CRa-Lsg-MS2

   Plasmid

   #247478

   Purpose

   sgRNA for INS activation cloned into LsgRNA-MS2 backbone

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Insert

   sgRNA targeting INS promoter (INS Synthetic, Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF

   Plasmid

   #200865

   Purpose

   AjTn6022_TniQ expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   AjTn6022_TnsF
   

   Expression

   Bacterial

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-601[insert(+1) TA-rich]

   Plasmid

   #221196

   Purpose

   601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   601[insert(+1)TA-rich]
   

   Use

   Unspecified

   Mutation

   601 has an additional mutation added 22 bp from d…

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304-HsTtyh1-TwinStrep-His

   Plasmid

   #211332

   Purpose

   Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Tweety homology protein 1
   

   Use

   Lentiviral

   Tags

   TwinStrep, 10xHis

   Expression

   Mammalian

   Mutation

   W417R

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-HsProm1s1-TwinStrep-His

   Plasmid

   #210816

   Purpose

   Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Tags

   TwinStrep, 10xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1-His6-E3(1)-CUbo-TwinStrep

   Plasmid

   #212712

   Purpose

   Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Tags

   6xHis and CUbo-TwinStrep

   Expression

   Bacterial

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDestTol2pA ins:Apollo-NADP+ mVenus

   Plasmid

   #210518

   Purpose

   Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Apollo-NADP+
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Tags

   mVenus

   Promoter

   Zebrafish insulin promoter

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS2409-Tier1-PhCMV-FURINsite

   Plasmid

   #169488

   Purpose

   Tier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Furin cleavage site
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCh-2xFKBP (no insert) (pBS1139)

   Plasmid

   #185322

   Purpose

   The empty vector used for the FKBP experiments in our paper

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

10. pG.Lantern-INS-SM50-GFP

    Plasmid

    #135594

    Purpose

    Propogation of Insulator-SM50 Promoter-GFP DNA for microinjection to visualize Insulator-GFP promoter activity in S. purpuratus

    Depositor

    Koji Akasaka, Mamiko Yajima

    Article

    Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

    Insert

    INS-SM50 promoter (SM50 )
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Green Lantern GFP

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Fusion of Zika NS2B-NS3 proteins

    Plasmid

    #204815

    Purpose

    Bacterial expression of a fusion of Zika NS2B-NS3 proteins

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Zika NS2B-NS3 fusion (orf1ab Zika)
    

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid

    #183790

    Purpose

    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Inserts

    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    

    Use

    Synthetic Biology

    Mutation

    G>A in the gRNA scaffold- please see depositor…

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-IRES-GFP/CALR ins5

    Plasmid

    #214701

    Purpose

    Mammalian expression of human CALR ins5

    Depositor

    Norio Komatsu

    Article

    Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

    Insert

    CALR ins5 (CALR Human)
    

    Use

    Retroviral

    Promoter

    MSCV

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pR0306 CcaCas13b His6-TwinStrep-SUMO

    Plasmid

    #182687

    Purpose

    Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    CcaCas13b
    

    Tags

    SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG001 His6-TwinStrep-SUMO-AapCas12b

    Plasmid

    #153162

    Purpose

    Bacterial expression plasmid for AapCas12b

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

    Insert

    AapCas12b
    

    Expression

    Bacterial

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINS-USP21 (USP, aa 196-565)

    Plasmid

    #61585

    Purpose

    Expresses human USP21 (USP domain) in E. coli.

    Depositor

    David Komander

    Article

    Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

    Insert

    USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Deleted aa 1-195.

    Promoter

    T7

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCD5-PR8-TEV-sfGFP-twinStrep

    Plasmid

    #157933

    Purpose

    Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

    Insert

    A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
    

    Tags

    sfGFP & TwinStrep

    Expression

    Mammalian

    Mutation

    E190D

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid

    #159283

    Purpose

    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Inserts

    Human INS sgRNA
    Human INS guide RNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PEA1-Puro-HEK3 CTT ins

    Plasmid

    #171996

    Purpose

    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Fusion of Dengue2 NS2B-NS3 proteins

    Plasmid

    #204792

    Purpose

    Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Dengue2 NS2B-NS3 fusion
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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