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pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

Plasmid

#199371

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT_tdTomato

Plasmid

#138567

Purpose

Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

Depositor

Hans Clevers

Publication

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

tdTomato

Use

Unspecified

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-H2B tdTomato

Plasmid

#116870

Purpose

Nuclear localized tdTomato

Depositor

Loren Looger

Publication

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Has service

AAV Retrograde

Insert

H2B tdTomato

Use

AAV

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-hA3A-BE3

Plasmid

#113410

Purpose

Expresses hA3A-BE3 in mammalian cells

Depositor

Jia Chen

Publication

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3 (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLX317-EGFP

Plasmid

#115439

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

EGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-EWB-DIO-cyan pre-eGRASP(p32)

Plasmid

#111589

Purpose

An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Promoter

Ef1a

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Opto-CRAC version 1

Plasmid

#101245

Purpose

Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

Depositor

Yubin Zhou

Publication

He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

Insert

LOV2 and STIM1 cytosolic fragment

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Publication

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Promoter

U6 and hsp70

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCW57.1-DUX4-WT

Plasmid

#99282

Purpose

All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

Depositor

Stephen Tapscott

Publication

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (DUX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Publication

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Publication

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pST44

Plasmid

#64007

Purpose

polycistronic cloning vector for pST44 plasmid suite

Depositor

Song Tan

Publication

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

Plasmid

#47457

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Tags

2A_GFP

Expression

Mammalian

Promoter

EF1-alpha

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDZ416 (24PP7SL loxP-Kan-loxP)

Plasmid

#45163

Depositor

Robert Singer, Daniel Zenklusen

Publication

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

24xPP7 Stem Loop Cassette

Use

Cre/Lox

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti6/V5-DEST-RNF2

Plasmid

#31216

Depositor

Lynda Chin

Publication

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

RNF2 (RNF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CMV500 empty vector

Plasmid

#33348

Depositor

Charles Vinson

Publication

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Publication

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

HisGST3C-lacZ

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGEX-2T-IQGAP1

Plasmid

#30107

Depositor

David Sacks

Publication

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Tags

GST

Expression

Bacterial

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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aMHC-Puro Rex-Blast

Plasmid

#21231

Depositor

Mark Mercola

Publication

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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