We narrowed to 10,838 results for: ENA

1. pBA409

   Plasmid

   #85971

   Purpose

   EF1a-sfGFP-Ubc-Neo

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sfGFP
   Neo
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF806-shRen.713

   Plasmid

   #186712

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   shRen.713
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVSVΔG-eGFP-linker

   Plasmid

   #231707

   Purpose

   Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

   Depositor

   Ron Geller

   Article

   Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

   Insert

   VSV antigenome
   

   Use

   Vsv expression

   Tags

   Expression

   Mammalian

   Mutation

   ΔG (VSV glycoprotein removed)

   Promoter

   T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-AAV-sh[control]

   Plasmid

   #75438

   Purpose

   control shRNA vector

   Depositor

   Edward Burton

   Article

   Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

   Insert

   GFP
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-p53/pRK5

   Plasmid

   #39237

   Depositor

   Xiaolu Yang

   Article

   Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-C1_SEPT9 i1

   Plasmid

   #71622

   Purpose

   mammalian expression of human SEPT9 with an mCherry fusion

   Depositor

   Elias Spiliotis

   Article

   Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

   Insert

   Septin 9 (SEPTIN9 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRa_VPR_yl

   Plasmid

   #107677

   Purpose

   CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

   Inserts

   Codon optimized dCas9-VPR
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK1010 (pSL25)

   Plasmid

   #191005

   Purpose

   Expresses blue chromoprotein on a broad host range RP4 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   blue chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-RIPK1

   Plasmid

   #78834

   Purpose

   To overexpress RIPK1 in Mammalian Cell

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

    Plasmid

    #125235

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs_FLAG-NMNAT1

    Plasmid

    #133259

    Purpose

    Express FLAG-tagged NMNAT1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT1 (NMNAT1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-EGFP-RhoA-wt

    Plasmid

    #12965

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSilencer2.1-U6-3JB1F+6

    Plasmid

    #190835

    Purpose

    For mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    3JB1F_T1+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E174R, R951K, R955A

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid

    #203671

    Purpose

    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

    Depositor

    Stephen Cusack

    Article

    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

    Insert

    MCES_VACCA_D1_D12
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

    Plasmid

    #204579

    Purpose

    lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEC-red

    Plasmid

    #196040

    Purpose

    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

    Depositor

    Paola Branduardi

    Article

    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Grna cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Plasmid EUPp_09

    Plasmid

    #157745

    Purpose

    PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

    Depositor

    Kang Zhou

    Article

    Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

    Inserts

    acetaldehyde dehydrogenase
    alcohol dehydrogenase (ADH2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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