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pX330A_D10A-1x5

Plasmid

#58775

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-hTRAF3 1-448

Plasmid

#44033

Depositor

Shao-Cong Sun

Publication

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 1-448; V274D and S393G

Available since

April 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSET FLIPE-1m

Plasmid

#13540

Depositor

Wolf Frommer

Publication

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207W in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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C1-MPAct(delta1-6)-mRuby3

Plasmid

#155223

Purpose

F-actin bidning mutant of MPAct

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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C1-FT-mCitrine-EEVEE-CaaX

Plasmid

#155225

Purpose

MPAct with increased linker flexiblity

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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C1-F-tractin-mCitrine

Plasmid

#155217

Purpose

Monitor global F-actin distribution

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Tags

mCitrine

Expression

Mammalian

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Publication

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOSV812

Plasmid

#126605

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV809

Plasmid

#126602

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV804

Plasmid

#126597

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV808

Plasmid

#126601

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV806

Plasmid

#126606

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1814_pCR8-gSMN2-DN2

Plasmid

#118647

Purpose

Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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E2-Crimson-FKBP(L,V)

Plasmid

#85000

Purpose

Expresses E2-Crimson-FKBP(L,V) in yeast

Depositor

Benjamin Glick

Publication

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

E2-Crimson-FKBP(LV)

Expression

Yeast

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Skp2 WT

Plasmid

#81117

Purpose

bacterial expression plasmid of Skp2

Depositor

Sung Hee Baek

Publication

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only
pIS1

Plasmid

#74887

Purpose

targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

Depositor

Klaus Foerstemann

Publication

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Inserts

Act5C upstream homology region
Act5C downstream homology

Tags

eGFP

Expression

Insect

Available since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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pZE31ms2

Plasmid

#25860

Depositor

Philippe Cluzel

Publication

Le et al Proc Natl Acad Sci U S A. 2005 Jun 28. 1

Insert

ms2 binding site (x2)

Expression

Bacterial

Mutation

none

Available since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only

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p7VP1

Plasmid

#24726

Depositor

Christopher Buck

Publication

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

VP1 from Human Polyomavirus 7

Expression

Mammalian

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHybrid-Electra1

Plasmid

#179474

Purpose

Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra1
mScarlet-I

Expression

Bacterial and Mammalian

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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