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pRMC2-msfGFP

Plasmid

#194913

Purpose

Tetracycline inducible expression of msfGFP in Staphylococci

Depositor

Rikke Meyer

Article

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno Sequence followed by monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Expression

Bacterial

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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HcKCR2_mCherry_pcDNA3.1

Plasmid

#177337

Purpose

Expresses KCR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR2

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-mCherry-NLS

Plasmid

#165442

Purpose

Expresses nuclear mCherry from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

mCherry-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.4

Plasmid

#111057

Purpose

Expresses dLight1.4 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.4

Tags

Flag tag

Expression

Mammalian

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P11

Plasmid

#120022

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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7h sgRNA for 4-μHOM reporter

Plasmid

#113625

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7h sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pC-HYG-YR

Plasmid

#61765

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Hygromycin
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pVC228 VEGF Site#3

Plasmid

#47507

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 3 (VEGFA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-FL

Plasmid

#34597

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
OPEN Beta2-microglobulin

Plasmid

#215657

Purpose

E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN Beta2-microglobulin (B2M Human)

Expression

Bacterial

Mutation

Histidine 31 to Cysteine

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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7i sgRNA for 4-μHOM reporter

Plasmid

#113626

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7i sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-Myr-Flag-IKBKE

Plasmid

#15293

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pKm-HCBE

Plasmid

#170333

Purpose

a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized CBEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBM019

Plasmid

#128179

Purpose

Construct for generating stable Cas9-expressing Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

Inserts

sgRNA#1
SpCas9
Chloramphenicol acetyltransferase

Use

CRISPR; Toxoplasma gondii expression

Tags

3X FLAG and Nuclear Localization Signal

Promoter

TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSB90 - pAGM4723_VirGN54D

Plasmid

#123187

Purpose

Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Type

Empty backbone

Use

Level 2 acceptor vector for expression in plants

Expression

Plant

Mutation

VirGN54D for increase virulence

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pOX + kqt3

Plasmid

#16138

Depositor

Larry Salkoff

Article

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-3 Nematode)

Use

Xenopus oocyte expression

Mutation

lacks aa1-16 compared to GenBank reference AY5729…

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pNP1047

Plasmid

#222726

Purpose

pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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ArpC4_pLib

Plasmid

#173681

Purpose

ArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC4 (ARPC4 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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Arp2_pLib

Plasmid

#173676

Purpose

Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp2 (ACTR2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pPD_Pinx-1::ChR2(H134R)::mCherry

Plasmid

#117420

Purpose

For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

channel rhodopsin-2 with H134R mutation

Expression

Worm

Mutation

H134R

Promoter

inx-1

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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