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Showing: 2981 - 3000 of 79172 results

  5. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74407-plasmid-map-sequence-id-140317

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  7. pcDNA3-PRB

    Plasmid
    #89130
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418
    Depositor
    Elizabeth Wilson
    Publication
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human progesterone receptor-B (PGR Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89130-plasmid-map-sequence-id-176077

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  14. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase HI-D10R-E48R
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60367-plasmid-map-sequence-id-456413

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Showing: 2981 - 3000 of 79172 results